THE GROWTH OF BACTERIOPHAGE
Emory L. Ellis and Max Delbrück
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http://jgp.rupress.org/cgi/content/abstract/22/3/365
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Lauren Kolski
ReplyDeleteEllis/Delbruck
1. The classic experiment in which they show that mutations arise randomly.
2. Just curious, why did you choose this article?
3. Random vs. induced mutation? Pros/Cons
Samat Kabani
ReplyDeleteEllis/Delbruck
1. The rate of adsorption of phage is proportional to the concentration of phage and to the concentration of bacteria and will burst independent of temperature.
2. How does this article help SyBBURE experiments?
3. Current methods of doing same experiment
Elizabeth Lillie
ReplyDeleteEllis/ Delbruck
1. The use of bacteriophage helped to determine different growth features in bacteria.
2. Is the d'Herelle plaque counting method still used or has it been replaced by something more accurate?
3. Similar experiments with the common cold virus
Amy Ostrowski
ReplyDeleteEllis/Delbruck
1. Bacteriophage growth is broken into latent phases and phases of exponential increase when the bacteria lyse.
2. Why was the plaque counting method unsatisfactory in other cases and why did it work well in their study? Is its accuracy dependent on the type of bacteriophage or bacteria?
Did they repeat this experiment using staph bacteria to verify the their results vs. the results found be Krueger/ Northrop?
3. Different types of bacteria and their affect on bacteriophage growth, is growth in all types similar to the one found by Ellis/Delbruck?
4. It's interesting that a simple mistake made by Krueger/Northrop in taking measurements at 30 minute intervals instead of smaller once can render significantly different results. I wonder how often this is occurring in experimentation today?
Patrick Diggins
ReplyDeleteEllis/Delbruck
1. The phage does not grow uniformly, but rather in in bursts.
2. Why was the plague counting method satisfactory for this paper, but not for other scientists? What would cause the bursts to vary in size?
3. The different effects of bacteriophages
Rosie Korman
ReplyDeleteEllis and Delbruck
1. This paper demonstrates that bacteriophages don't grow - they replicate inside of a bacterium and then are released all at once.
2. How were viruses thought to replicate before this paper?
Was this the first instance of use of an anti-E. coli bacteriophage?
3. Retroviral replication
1. This paper demonstrates several methods in quantification of the micro-epidemiological factors of a strain of E coli. Using rate comparison, the author shows that there are several proportional factors, such as the proportionality between adsorption of phage and bacteria present, functions for the three periods of phage growth, and an indifference in burst time between a bacteria infected by single and multiple phages.
ReplyDelete2. What is d'Herelle's method? Can these proportions be used to quantify the amount of virus present in a human as well? If a virus is ever utilized in medicine as an anti-cancer agent, (http://www.medicineatyale.org/v4i4_sept_oct_2008/brain.html), will these figures help determine the amount of virus to use?
3. Methods in viral medicine using these concepts
Rachel Harvey
ReplyDeleteEllis/Delbruck
1. The growth of phages can be observed in bursts of varying magnitudes, due to external and internal factors.
2. Have there been any improvements upon these experimentation methods?
3. Growth spurts of phages
1. Phages replicate inside bacteria and are then released to infect more bacteria.
ReplyDelete2. What is the difference between an infective center and a plaque?
3. What else has been discovered about phages since this paper was published
Parker Gould
ReplyDeleteDelbruck and Ellis
1. The cycle of growth for a phage has three main steps: attachment, latent growing period, and bursting. That they grow inside and then burst is probably most significant.
2. What is this d'Herelle's method for counting phages?
3. Other landmark papers in science.
4. It's pretty cool that d'Herelle predicted the results of something like this 12 years before it was proven (or at least that's how I read it).
Chaitanya Allamneni
ReplyDeleteEllis & Delbruck
1. Bacteriophages (essentially viruses that infect a bacteria) growth within a bacterium and "burst" at once.
2. What is the current method for phage counting
3. Different types of bacteria/phages and their growth
Tommy Byrd
ReplyDeleteDelbruck and Ellis
1. Early studies into viral replication revolved around analysis of virion-induced plaques on bacteria cultures from bacteriophage particles
2. Did Delbruck and Ellis ever propose a detailed description of the bacteriophage lifecycle or speculate on such a process?
3. Viral methods of infection, especially bacteriophages compared to viruses that infect eukaryotes.
4. This study, in retrospect, seems to indirectly suggest the possibility of plasmid vector cotransfection, which is used so commonly and with such impressive results in contemporary biomolecular science. Amazing scientific methodology.
Hussain Jinnah
ReplyDeleteEllis & Delbruck
1. Bacteriophages replicate within a bacterial host and are released in explosive bursts.
2. Interesting article (and historically relevant), but why did you pick it for SyBBURE?
3. You could go over the lytic and lysogenic cycles of viral amplification within a host.
Liwei Jiang
ReplyDeleteEllis and Delbrück
1. Learned: Bacteriophages follow a growth curve characterized by plating, adsorption, and bursting rates.
2. Pressing ?: Has any of his findings been proved wrong?
3. Presentation: Walk through the bacteriophage life cycle.
4. Thoughts: I got this for my father's birthday last year. (He is an immunologist.)
Ayeeshik Kole
ReplyDeleteEllis and Delbruck
1. Bacteriophage growth can be separated into three distinct stages of adsorption of the phage on the bacterium, growth on the bacterium, and the bursting of the phage.
2. How was the d'Herelle plaque counting method considered unsatisfactory by some investigators?
How was this publication initially received?
3. The flaws with Kreuger and Northrop's measurements
Jake Brady
ReplyDelete1.) Bacteriophage growth is performed not by normal replication but by absorption by bacterium, growth of of the phage using bacterial cellular mechanisms, then the lyse of the bacterium releasing more phage.
2.) What is the d'Herelle plaque counting method?
3.) the differences in growth cycles of different strains of bacteriophage.