Friday Journal Club article and supplement

Here are the article and supplement, hope it works.

http://www.nature.com/nature/journal/v446/n7131/pdf/nature05561.pdf
http://www.nature.com/nature/journal/v446/n7131/extref/nature05561-s1.pdf

sybbure journal club 11/17

here's the link to the article...hope it works

this link is to the pdf

http://nanoscience.bu.edu/papers/Biosensors%20and%20Bioelectronics_Sept%202006.pdf

SyBBURE Journal Club 11/13/09

Not sure if this will work, but here's a link to the article.

http://docs.google.com/a/vanderbilt.edu/fileview?id=0B8tdOqHX4KclZmQxOGJhNDMtMzExMy00ZjZlLWI3Y2QtN2FhMzg4NjI3Zjgx&hl=en

PCR 11-3

Max Housman
Cheng
1. This is a microfluidic device that is capable of separating out T cells from whole blood by affinity chromatography in a simple, low budget setting.
2. While they plan on making this low budget and affordable, how feasible is it to have a microscope in a low budget clinic. This seems like it would make quantification a large challenge.
3. Other methods of quantification other than a microscope, like an automated chip based on fluorescent readings or something. Maybe some other way to collect, label, and quantify captured cells other than a scope.

For some reason I couldn't post this under the blog for the journal club. It is still acting strange for me and I don't know why. This is the only place it would let me post.

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Tommy Byrd
A microfluidic device for practical label-free CD4+ T cell counting of HIVinfected
subjects
Cheng
1. Microfluidic devices can be useful for immobilizing cells counting on antibody layers for counting, in this case to diagnose HIV progression in T cells
2. Is the distribution of CD4+ T cells equal throughout the human circulatory system in HIV infected patients? Is there any concern that using such a small volume of blood could give skewed, unrepresentative results?
3. Current methods for HIV testing in poor countries, immunohistochemistry description
4. I wonder if there might be some way to count the cells in a mechanical way rather than by microscopic observation, so that almost no training would be needed to use this testing device

Oct 30 Journal Club

Peter DelNero
Sowinski
1) I learned about T-cell membrane nanotubes, and how they could play a role in intracellular transmission of HIV-1. These structures appear to accelerate transmission over long distance.
2)What would be the side-effects of targeting membrane nanotubes in HIV therapy?
3)How intracellular transport affects virulance- compare diffusion to other pathways
4)It seems very frightening that HIV can use T-cell nanotubes to rapidly spread. It's like an viral interstate. I'm curious about what else these nanotubes could be used for.

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Journal Article for Tuesday Oct 20.

Hi guys!
Sorry for putting this post up so late.
Monitoring Dynamic Changes in Lymph Metabolome of Fasting and Fed Rats by Electrospray Ionization-Ion Mobility Mass Spectrometry (ESI-IMMS)
Kimberly Kaplan, Prabha Dwivedi, Sean Davidson, Qing Yang, Patrick Tso, William Siems and Herbert H. Hill, Jr.
Anal. Chem., 2009, 81 (19), pp 7944–7953

Formatting your text in comments

If you want to include some text formatting in the blog comments, read the following:

http://mike.brisgeek.com/2006/08/30/simple-html-for-formatting-blogger-comments/

Don't Worry about Math

Hey guys, if you're slaving over the math, don't worry about it, we're not going to go into great detail about it. It is merely discussing how the simulation is formed, and we're really interested in the results of the simulation. So don't worry about it, focus on the other figures.

HK

Journal Article for Oct. 16

The article is Electroosmotic flow analysis of a branched U-turn nanofluidic device, Gea O. F. Parikesit, Anton P. Markesteijn, Vladimir G. Kutchoukov, Oana Piciu, Andre Bossche, Jerry Westerweel, Yuval Garinia and Ian T. Younga. Lab Chip, 2005, 5, 1067-1074.

The link is: http://www.ph.tn.tudelft.nl/People/albert/papers/05.Nanophoresis_LoC.pdf.

You know the drill.

PDMS bonding

Sorry I'm late. I forgot to check my SyBBURE inbox before today.

Topic: PDMS bonding techniques

Learned: Variety of considerations for manufacturing PDMS- experiment designed to optimize bonding strategy

Questions: When is the microwave bonder going to work in our microfab room?
are there many strategies for bonding not addressed in the paper?

Topics: scale-up considerations for PDMS manufacturing

Name: Peter DelNero

Article for Tuesday, September 29

Eddings, M.A., Johnson, M.A. & Gale, B.K. Determining the optimal PDMS-PDMS bonding technique for microfluidic devices. Journal of Micromechanics and Microengineering 18, 067001 (2008).

URL: http://iopscience.iop.org/0960-1317/18/6/067001/?ejredirect=.iopscience

Please complete your PCR's by 8 PM on Monday, September 29.

Thanks,

Liwei and Elizabeth

PCR

Samat Kabani

Microcontact Printing of Proteins by Andre Bernard

1) I learned that this technique of microprinting of proteins has the advantage of being able to process large bioactive surfaces in one run and with arbitrary patterns.

2) What are some specific examples of where this process was used and how much better it was compared to standard techniques?

3) The importance of Microcontact Printing in modern research

September 25 PCR

Elizabeth Lillie
So I have no idea if I'm doing this right but here is my attempt at posting my pcr:



1.) Learned about Microcontact printing of Proteins and the advantages of this technique over previous methods.
2.) This might be a dumb question, but what exactly does printing proteins on subtrates do, how do they work, like in the applications mentioned in the introduction?
3.) Application of microcontact printing in a more specified bioassay

Journal article for 9/15

Hey guys sorry I didn't post this at the same time as sending out the email.
the link is to the find it at VU page:

http://sfx.library.vanderbilt.edu/vu?&url_ver=Z39.88-2004&url_ctx_fmt=info:ofi/fmt:kev:mtx:ctx&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Automated%20patch-clamp%20technique%3A%20Increased%20throughput%20in%20functional%20characterization%20and%20in%20pharmacological%20screening%20of%20small-conductance%20Ca2%2B%20release-activated%20Ca2%2B%20channels&rft.auinit=R&rft.aulast=Schroder&rft.date=2008&rft.epage=647&rft.genre=article&rft.issn=1087-0571&rft.issue=7&rft.spage=638&rft.stitle=J%20BIOMOL%20SCREEN&rft.title=JOURNAL%20OF%20BIOMOLECULAR%20SCREENING&rft.volume=13&rfr_id=info:sid/www.isinet.com:WoK:UA&rft.au=Friis%2C%20S&rft.au=Sunesen%2C%20M&rft.au=Mathes%2C%20C&rft.au=Willumsen%2C%20N&rft_id=info:doi/10.1177%2F1087057108320274

Sorry I can't get the link to work right but if you copy and paste it'll take you there. So I also can't figure out how to fully delete my other post, any suggestions?

Again 10-11 for pcr, I'd love you guys.

SyBBURE JC Article 2009_09_11

This week's article will be "The Perverse Effects of Competition on Scientists’ Work and Relationships" Melissa S. Anderson, Emily A. Ronning, Raymond De Vries, Brian C. Martinson
Sci Eng Ethics (2007) 13:437–461 DOI 10.1007/s11948-007-9042-5