Tommy Byrd
A microfluidic device for practical label-free CD4+ T cell counting of HIVinfected
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Cheng
1. Microfluidic devices can be useful for immobilizing cells counting on antibody layers for counting, in this case to diagnose HIV progression in T cells
2. Is the distribution of CD4+ T cells equal throughout the human circulatory system in HIV infected patients? Is there any concern that using such a small volume of blood could give skewed, unrepresentative results?
3. Current methods for HIV testing in poor countries, immunohistochemistry description
4. I wonder if there might be some way to count the cells in a mechanical way rather than by microscopic observation, so that almost no training would be needed to use this testing device
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Hussain Jinnah
ReplyDeleteCheng
1. For resource-limited settings, simple microfluidic devices can be used to monitor HIV disease progression via counting of CD4 T lymphocytes.
2. Can you explain differential shear flow?
3. Actual cost of using this method vs conventional techniques.
Niki Arinze
ReplyDeleteCheng
1. Differences in shear stress and flow can be applied to sort cells
2. How does the flow cytometry work, since it does not deplete cells from whole blood?
Why do they divide by 10 to get the CD4 Count in their device
3. The practicality of using microfluidic medical diagnostics in limited resource areas
Samat Kabani
ReplyDeleteCheng
1. Inexpensive-to-make microfluidic devices can use flow properties to count CD4+ cells to diagnose HIV.
2. Have these devices been utilized on a large scale yet?
3. The future of microfluidics in disease diagnostics
Liwei Jiang
ReplyDeleteCheng
1. Learned: A simple microfluidic device using affinity chromatography to bind CD4+ T cells in blood is useful for doing cell counts in poor settings.
2. Pressing ?: Intuitively, what is shear stress, and how does it affect cell sorting?
3. Presentation: Comparison: flow cytometry vs. microfluidic method
4. Thoughts: A practically identical paper from the same authors was presented last year by Laura Wertz. It is refreshing to see the subject come up once more.
Elizabeth Lillie
ReplyDeleteCheng
1.) Microfluidic devices can be used to count CD4+ T lymphocytes and diagnose HIV disease staging.
2.)How precise is the method of binding the cells to the device, does it consistently attach the same percentage of cells.
3.)Other methods of measuring CD4+ levels
Peter DelNero
ReplyDeleteCheng
1) The article talks about using an affinity chromatography column to separate CD4 cells for HIV diagnosis in low-resource areas.
2) How easy is it to create the differential shear flow conditions that they used? How often is shear stress combined with affinity columns for cell separations?
3) HIV diagnostics / conditions in low-resource areas
4) Sounds like a good device. Funny that the same topic came up last year with Wertz- hope they weren't too identical. These guys made it sound like they were the first.