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JOURNAL CLUB BLOG

Monday, November 9, 2009

SyBBURE Journal Club 11/13/09

Not sure if this will work, but here's a link to the article.
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7 comments:

  1. PatrickNovember 12, 2009 at 2:21 PM

    Patrick Diggins IV
    Torisawa

    1. Through the use of micro-fluidic channels, embryoid bodies of consistent size can be created and their size is dependent on the cross-sectional size of the microchannels.

    2. Why is it important to have EBs of different sizes?

    3. From embryoid bodies to neuron cells

    4. Is there a danger of damaging cells when they are harvested from the microchannels.

    ReplyDelete
  2. Elizabeth LillieNovember 12, 2009 at 2:52 PM

    Elizabeth Lillie
    Torisawa
    1. Microfluidic channels were used for synchronized formation of uniform size embryoid bodies.
    2.Does the EB size effect what the ES cells can differentiate into?
    3. Papers with deferent contraints on stem cells to control their differenciation.

    ReplyDelete
  3. tesniemNovember 12, 2009 at 9:09 PM

    Tesniem Shinawi
    Torisawa
    1.A method using a microfluidic system allowed the straightforward synchronized formation of uniform-sized embryoid bodies.
    2. How does the compartmentalization of the membrane affect the EBs and what is the effect or importance of their size?

    ReplyDelete
  4. Ayeeshik KoleNovember 12, 2009 at 10:24 PM

    Ayeeshik Kole
    Torisawa

    1. A microfluidic system can be used to control the size and formation of EBs and subsequent ES cell processing.
    2. Does the size of the microchannels ever become an issue during further differentiation or culture?
    3. The importance of size of EBs

    ReplyDelete
  5. ParkerNovember 12, 2009 at 11:25 PM

    Parker Gould
    Torisawa

    1. You can use a uFD to create uniformly-sized embryoid bodies of stem cells.
    2. How did they introduce the cells to the side-chambered device? Individual populations to each chamber?
    3. Growing ES EBs with droplet microfluidics.

    ReplyDelete
  6. Tang DhummakuptNovember 12, 2009 at 11:53 PM

    Tang Dhummakupt
    Torisawa

    1. You can use microfluidics to control the formation of embryoid bodies

    2. What's the reason for using the cuboidal shapes as opposed to the straight channel?

    3. Stem cell differentiation

    ReplyDelete
  7. Chaitanya AllamneniNovember 13, 2009 at 3:12 PM

    Chaitanya Allamneni
    Torisawa

    1. Microfluidic system can be used to synchronize the formation of embryoid bodies of specific sizes. The central goal is to regulate EB size.

    2. They are more concerned with channels instead of traps, like our devices. Have you considered placing EB's in with channels such as these, instead of square-shaped traps?

    3. As far as EB size, at what stage and size do the the EB's differentiate into cardiomyocytes. Do EB's at diferentiate stages have the capability to differentiate into different cells?

    ReplyDelete

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