this link is to the pdf
Sunday, November 15, 2009
Monday, November 9, 2009
Monday, November 2, 2009
PCR 11-3
Max Housman
Cheng
1. This is a microfluidic device that is capable of separating out T cells from whole blood by affinity chromatography in a simple, low budget setting.
2. While they plan on making this low budget and affordable, how feasible is it to have a microscope in a low budget clinic. This seems like it would make quantification a large challenge.
3. Other methods of quantification other than a microscope, like an automated chip based on fluorescent readings or something. Maybe some other way to collect, label, and quantify captured cells other than a scope.
For some reason I couldn't post this under the blog for the journal club. It is still acting strange for me and I don't know why. This is the only place it would let me post.
Cheng
1. This is a microfluidic device that is capable of separating out T cells from whole blood by affinity chromatography in a simple, low budget setting.
2. While they plan on making this low budget and affordable, how feasible is it to have a microscope in a low budget clinic. This seems like it would make quantification a large challenge.
3. Other methods of quantification other than a microscope, like an automated chip based on fluorescent readings or something. Maybe some other way to collect, label, and quantify captured cells other than a scope.
For some reason I couldn't post this under the blog for the journal club. It is still acting strange for me and I don't know why. This is the only place it would let me post.
Sunday, November 1, 2009
Tommy Byrd
A microfluidic device for practical label-free CD4+ T cell counting of HIVinfected
subjects
Cheng
1. Microfluidic devices can be useful for immobilizing cells counting on antibody layers for counting, in this case to diagnose HIV progression in T cells
2. Is the distribution of CD4+ T cells equal throughout the human circulatory system in HIV infected patients? Is there any concern that using such a small volume of blood could give skewed, unrepresentative results?
3. Current methods for HIV testing in poor countries, immunohistochemistry description
4. I wonder if there might be some way to count the cells in a mechanical way rather than by microscopic observation, so that almost no training would be needed to use this testing device
A microfluidic device for practical label-free CD4+ T cell counting of HIVinfected
subjects
Cheng
1. Microfluidic devices can be useful for immobilizing cells counting on antibody layers for counting, in this case to diagnose HIV progression in T cells
2. Is the distribution of CD4+ T cells equal throughout the human circulatory system in HIV infected patients? Is there any concern that using such a small volume of blood could give skewed, unrepresentative results?
3. Current methods for HIV testing in poor countries, immunohistochemistry description
4. I wonder if there might be some way to count the cells in a mechanical way rather than by microscopic observation, so that almost no training would be needed to use this testing device
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