Hey guys sorry I didn't post this at the same time as sending out the email.
the link is to the find it at VU page:
http://sfx.library.vanderbilt.edu/vu?&url_ver=Z39.88-2004&url_ctx_fmt=info:ofi/fmt:kev:mtx:ctx&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Automated%20patch-clamp%20technique%3A%20Increased%20throughput%20in%20functional%20characterization%20and%20in%20pharmacological%20screening%20of%20small-conductance%20Ca2%2B%20release-activated%20Ca2%2B%20channels&rft.auinit=R&rft.aulast=Schroder&rft.date=2008&rft.epage=647&rft.genre=article&rft.issn=1087-0571&rft.issue=7&rft.spage=638&rft.stitle=J%20BIOMOL%20SCREEN&rft.title=JOURNAL%20OF%20BIOMOLECULAR%20SCREENING&rft.volume=13&rfr_id=info:sid/www.isinet.com:WoK:UA&rft.au=Friis%2C%20S&rft.au=Sunesen%2C%20M&rft.au=Mathes%2C%20C&rft.au=Willumsen%2C%20N&rft_id=info:doi/10.1177%2F1087057108320274
Sorry I can't get the link to work right but if you copy and paste it'll take you there. So I also can't figure out how to fully delete my other post, any suggestions?
Again 10-11 for pcr, I'd love you guys.
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Liwei Jiang
ReplyDeleteSchrøder
1. The gist of the article is that an automated patch clamp technique is suitable for measuring activity of the CRAC channel.
2. Could someone explain in plain words how the patch clamp method works? Specifically, how does the measured current correspond to the ion channel activity?
3. A brief overview of patch clamp techniques in general.
4. Stephen, does your research involve a patch clamp technique for measuring ion channel activity?
Tesniem Shinawi
ReplyDeleteAutomated Patch-Clamp Technique: Increased Throughput in Functional Characterization and in Pharmacological Screening of Small-Conductance Ca2+ Release-Activated Ca2+ Channels
SCHRØDER
CRAC channels can be targeted with automated patch-clamp techniques in an efficient way to determine the characterization of physiological and pharmalogical properties.
Can you briefly explain the significance of the CRAC channel and how it functions, in simple terms. How is CRAC specifically related to pharmacological agents and immunology and how can it actually affect these fields?
Hussain Jinnah
ReplyDeleteWHAT IS THE SINGLE MOST IMPORTANT THING THAT YOU LEARNED IN THE READING?
-The patch-clamp technique can be used to measure a specific cell current called a CRAC current.
WHAT QUESTIONS DO YOU HAVE BASED ON THE READING?
-Can this technique be extended to study neurons?
SUGGEST A TOPIC FOR A REPORT/PRESENTATION BASED UPON THE READING.
-Does anyone within VIIBRE/SyBBURE use the patch-clamp method in their research?
Niki Arinze
ReplyDelete1.Patch clamp technology can be used to target and study CRAC channels
2. I second Liwei's question, How do patch clamps work, especially for whole cell studies? Is there a way to do single channel studies?
3. Topic- A comparison of technologies for studying voltage gated channels.
Patrick Diggins
ReplyDeleteSchrøder
1. An automated patch clamp not only can be used in the study of CRAC channels but has advantages stemming from the improved reproducibility and consistent analysis of data by the software.
2. What is the function of a CRAC channel?
3. Causes of immunodeficiency disorders.
Tommy Byrd
ReplyDeleteSchroder
1. Learned
-QPatch is an effective method to detect cellular voltage gradients in response to drugs
2. Pressing Questions
-Why was it difficult to get high-resistance seals if cell culture confluency was much lower or higher than 80%?
3. Presentation
-Visual depiction/description of the QPatch experimental setup
4. Thoughts
-Do the CRAC immunodeficiency disorders mentioned by the authors occur because abnormal transmembrane ion concentration gradients cause cell death?
Chaitanya Allamneni
ReplyDeleteSchroder
1. Automated patch clamp method, named QPatch in this experiment, is an effective means to measure of voltage/current within a channel (specifically, the CRAC channel). To measure voltage levels, calcium stimulants were applied to the CRAC channel.
2. What are the primary functions of the CRAC channel? The author says it plays a role in Alzheimers and immunodeficieny disorders, but how so? Also, what are examples of disorders involving store-operated calcium channels.
3. Have any previous links been determined between immunodeficiency disorders and calcium channel dysfunction, etc? Can any other ions pass through the CRAC channel?
4. I remember studying the conventional patch clamp method in Neuroscience, can this automated one also be used to study neurons in the brain. That would be critical i think.
Sami Ayad
ReplyDeleteSchrøder
1. Learned
Development of an automated patch-clamp technique to study CRAC channels in response to calcium activation of cells using different substances (i.e. ionomycin).
2. Pressing Questions
-The neonatal Immune System is known to be very weak in comparison to the adult ones. Could you say that the reason why their immune response is weaker is due to impaired CRAC channels or calcium flux in either B or T lymphocytes?
3. Presentation
Other methods used to characterize CRAC channel properties.
Ayeeshik Kole
ReplyDeleteSchrøder
1. The QPatch system is precise in measuring CRAC currents both rapidly and effeciently and can be utilized for pharmalogical studies.
2. How exactly does it work and how were they able to achieve a good signal to noise ratio?
3. The importance of automated patch clamp technology in devoloping immune response drugs