Hiroshi Kurosawa
http://linkinghub.elsevier.
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
JOURNAL CLUB BLOG
Posts
Welcome to the SyBBURE Journal Club
Rules:
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
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Rachel Harvey
ReplyDeleteKurosawa
1. Different methods of EB formation will produce different results, and should be chosen accordingly.
2. What does LIF do?
3. Appropriate Culture Techniques for EBs
4.It is interesting that in the spinner flask method, different rpm will induce differential growth.
Niki Arinze
ReplyDeleteKurosawa
1. Of the current methods being used for the formation of embryoid bodies, there is little homogeneity in results?
2. Why would Leukemia inducing actor prevent spontaneous differentiation?
3. investigation of the effect of the variation of EB on experimental results
4. It seems like there's real way to have repeatability or reproducibility in this field
Patrick Diggins
ReplyDeleteJurosawa
1. Little attention is placed in the quality of EBs and the different protocols used to induce EB formation in papers at the moment.
2. How spontaneous is the differentiation of ES cells in the absence of LIF or MEF feeder layers?
3. Examples of when different techniques would be better than the others
4. What is the role of probability or chance in the differentiation of the ES cells?
Ayeeshik Kole
ReplyDeleteJurosawa
1. Most EB production protocols have been developed on an individual basis, and this outlines the benefits and disadvantages related to each method.
2. How successful is creating homogenous EBs on a microfluidic platform?
3. The problems with not implementing standard EB formation protocols in research
1. There are different methods for producing embryoid bodies in vitro, and each method yields different results.
ReplyDelete2. How does LIF prevent differentiation?
3. Possible examples concerning how different techniques of EB formation has produced different results for similar experiments.
Tommy Byrd
ReplyDeleteJurosawa
1. Each method of culturing EBs has certain advantages and disadvantages; at this point researchers need to uncover how each method affects the EBs particular genotype.
2. Where do most human stem cells come from? Are they primarily from preexisting lines, discarded embryos, or differentiated cells that have been converted back to an undifferentiated state?
3. Specific applications for EB culture in research and medicine
Elizabeth
ReplyDeleteJurosawa
1. There are several different methods to induce the differentiation of embrionic stem cells into 3 different embryoid bodies (ectoderm, endoderm, mesoderm).
2. Are all ES cells in research initially pluripotent? Why not start with totipotent ones because they are still totipotent in the blastocyst which is from where the stem cells are acquired.
3. Successful medical applications of ES/EB cells
Liwei Jiang
ReplyDeleteKurosawa
1. Learned: different ways to form EBs. Homogeneity seems to be the most important factor.
2. Pressing ?: Do the scalable production methods suffer in homogeneity compared to the laboratory, low-volume methods?
3. Presentation: Summarize Figure 3.
Amy Ostrowski
ReplyDeleteKurosawa
1. There are several different methods used to produce EBs, however there are andvantages and disadvangtes to each. It is important to determine a protocol which will consistently produce a large number of EBs in a controlled manner.
2. Why is LIF effective in mouse cells but not in human cells?
3. Examples of EBs used in research today and how incorrect culturing can affect results.
Stephen Arndt
ReplyDeleteKurosawa
1. different methods of EB formation from cultured totipotent stem cells, but there are problems with homogeneity
2. How do antidifferentiation factors work and how does one regress a cell back to an undifferentiated state from a more differentiated state. Also if you clone a clone does it age even faster than the original clone?
3. presentation: where stems cells were supposed to be in 2010 as predicted in 1990 and 2000
1. There are different methods for EB formation, and this has an important effect on the quality and homogeneity of stem cells.
ReplyDelete2. What is a feeder layer? How do you make one?
3. The obstacles currently preventing stem cell therapy from being a viable medical procedure.
Samat Kabani
ReplyDeleteKurosawa
1. There are several different methods of culturing EBs that producing varying quality and homogeneity.
2. Have these methods been utilized by in SyBBURE? If so, which one(s)?
3. Necessity of controlled procedure when working with stem cells
Rosie Korman
ReplyDeleteKurosawa
1. There are severaldifferent methods for forming EBs, and each method has its specific advantages and disadvantages.
2. What happens if an EB is allowed to grow indefinitely?
Can human EBs be cultured on mouse feeder layers?
3. Tissue types that can be produced by differentiated EBs.
4.
Peter DelNero
ReplyDeleteKurosawa
1. This article provided a review for methods of forming EBs. I did not know what an EB was before reading this article.
2. Would this type of research on human embryonic stem cells be legal in the U.S. in 2007? Is it legal now? Why is it controversial?
3. History of the development of embryonic stem cell research and the controversy surrounding it
4. I thought it was interesting that you chose a review of literature as the journal article. I like that it provides a broader perspective on methods, but it also did not offer as much insight into the specific challenges that can be found in a research paper.
Chaitanya Allamneni
ReplyDeleteKurosawa
1. This article covers the different methods for culturing embryoid bodies, specifically the suspension culture, methylcellulose culture, and hanging drop (which you are doing?)
2. I noticed that the article mentioned that embryonic stem cells were pluripotent. Why are they not totipotent? Which cell types can EB's not differentiate into? At which stage do the EB's begin to differentiate.
3. Functionality of cardiomyocytes differentiated from embryoid bodies; various factors that induce differentiation of EB's