Building a better cell trap: Applying Lagrangian modeling to the design of microfluidic devices for cell biology
Min-Cheol Kim
http://jap.aip.org.proxy.library.vanderbilt.edu/japiau/v103/i4/p044701_s1?view=fulltext
Subscribe to:
Post Comments (Atom)
Posts
Stephen Arndt
ReplyDeleteBuilding a better cell trap: Applying Lagrangian modeling to the design of microfluidic devices for cell biology
Min-Cheol Kim
1. U shaped cell traps allow specific numbers of trapped cells in assumed good micro environments
2. with constant perfusion, isn't this device going to constantly eliminate any produced growth factors? And without it the cells would exhaust nutrients even more quickly than in culture? What is the flow profile over a U trap? totally diffusion driven?
3. Cell traps- now and then
Lauren Kolski
ReplyDeleteKim
1. They developed a simulation method to model the trapping of cells
2. What is Computational fluid dynamics simulations?
3. Single cell in traps vs. small groups vs. large aggregates. Pros/Cons
Elyse Sadeghi
ReplyDelete1.Validated simulation method to model cell trapping
2.What is Lagragian modeling?
3.Current research related to growing cell culture in microfluidic devices
Rachel Harvey
ReplyDeleteKim
1. A modeling method was developed to simulate the trapping of cells in microfluidics in different sieve structures.
2. Can you elaborate on the Lagrangian approach/modeling?
3. Efficacy of different sieve structures for cell trapping
Liwei Jiang
ReplyDeleteKim, Min-Cheol
1. Learned: The authors have developed a computational method that is pretty good at modeling the locations of cells in microfluidic devices.
2. Pressing ?: (1) What are weir-type filters? (2) What is the finite volume method? (3) What is the definition of "aperture" in the context of this paper?
3. Presentation: What does it mean to have low Reynolds number?
4. Thoughts: How good is COMSOL compared to what the authors are using?
Amy Ostrowski
ReplyDeleteKim
1. A simulation of trapping cells in sieves was modeled and then verified experimentally.
2. What is the Lagrangian method? How long do the cells remain in the device?
3. Applications for the usage of a such a device, cell signaling?
Parker Gould
ReplyDeleteKim
1. Simulations and experimental data from various cell traps were compared.
2. Is there any big difference in the modeling as the size trends downward and gets closer to the T-cell trap size? (e.g. any laminar effects?)
3. Is the source code for LCD available?
Peter DelNero
ReplyDeleteKim
1. The investigators used a lagrangian approach to develop a model for predicting the number of cells per trap in various geometries
2. Please explain how the Lagrangian method works and how it allowed them to create the model
3. Advantages and disadvantages of various trap geometries
4. Maybe consider some of the other specifications that might influence cell trapping besides geometry- like surface coatings, channel height, types of cells, etc.
1. A method was developed to model the trapping of cells within a microfluidic device.
ReplyDelete2. It was asked above, but I too would like an explanation of Lagrangian moedline.
3. Is the number of cells trapped per sieve trapped relatively constant, or does it vary greatly.