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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
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Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
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Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
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An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Kevin Roman
ReplyDeletePark
1. A hight of only 1um is enough to keep cell in the well when the device is being flushed.
2. How long can the cell survive since the media is drying up with time in the wells?
3. A presentation could be given to compare the capture rate between traps and wells.
Rachel Harvey
ReplyDeletePark
1. A receding-meniscus method can be used to passively trap yeast in MF devices, and subsequent experiments can be performed on the yeast.
2. It may just be a matter of fluid dynamics, but when the MF is washed with water to remove excess yeast, how do the yeast and media stay in the wells? Are some washed away but not all?
Does water have any negative effects on yeast?
Why is fluorescent distribution and intensity different from cell to cell?
3. Passive Yeast isolation in MF devices
Pendell Meyers
ReplyDeletePark
1. Because of the fluid dynamics of the receding meniscus and the size of the 10 micrometer wells, there is a minimum density of 1.5x10^7 cells/mL to ensure uniform cell docking.
2. Even if the media does not evaporate significantly, how long can the cells survive in such a small volume of stagnant media?
3. Would aspirating the media back out of the entrance port produce the same cell docking as evaporation? If so, it would be faster and there would be more media remaining in the wells.
Samat Kabani
ReplyDeletePark
1. Use the receding meniscus method and capillary action, yeast cells were able to be trapped in microwells on an individual basis.
2. Once the cells are in the microwells, is it possible for the yeast cells to migrate out of it? As the cells divide and fill the microwell, how does on prevent the cells from coming out?
3. Yeast growth in a MF device
Alex Garza
ReplyDeletePark
1. Another MF device to trap cells that works by using a receding meniscus. Usually able to trap cells individually and in this experiment, were testing the affect of the alpha mating on yeast.
2.With such a small depth for the wells, would it be possible to the cells to be pushed out? Also, how long can the cells live in such a small area with this media?
3.Comparison of different MF devices and the results obtained from trapping cells in the different devices.
Lauren Kolski
ReplyDeletePark
1. A MF device for the capture of non-adherent yeast in wells using a receding meniscus and lateral capillary force.
2. Can you expound upon this "lateral capillary force"? How is it formed?
3. Pros and cons of this device vs. the usual cell traps.
1. A microfluidic device that can be used to passively capture yeast cells using evaporation and capillary flow, where cells are deposited into 1 um wells by a spontaneously formed receding meniscus.
ReplyDelete2. After the cells are deposited into the 1 um wells, can they be bathed in different media without being washed away? Also, this may be a stupid question, but what activity is the GFP measuring? Does it mark cells that are actively budding and dividing?
3. A presentation could be given about the benefits of using systems that passively trap cells vs. ones that don't, also the benefits of isolating single cells or small groups of cells for study
This comment has been removed by the author.
ReplyDeleteTrevan Locke
ReplyDeletePark
1. This MF device uses wells and a receding meniscus to capture one or multiple yeast cells for further study.
2. Why would this method of trapping in wells be better than the typical trap device that allows fresh media to continue flowing?
3. Comparison with other trapping devices.
1.Can capture non-adherent yeast cells in microwells by using a receding meniscus method that is simple and cheap.
ReplyDelete2.What are the benefits of using a 1 um well as opposed to the 60 um height?
3.How would this method of capturing non-adherent yeast cells improve our own research projects at SyBBURE?
1. Using a receding-meniscus method, yeast cells can be captured in microwells afterwhich various studies may be made on the captured cells.
ReplyDelete2. How can the method be improved so that the cells can be bathed in a variety of solutions without being washed out of the microwells? What is the time-frame the cells can be studied once captured into the microwells? What makes this method better than others?
3. Improvements in the device and comparisons to others.
Jake
ReplyDeletePark
1.) using the receding meniscus formed through evaporation, yeast cells are trapped in micro-wells.
2.) what are the advantages of this method over traditional, continuous flow trap methods?
3.) example experiments that could be efficiently performed in this device and not in others.
Ayeeshik Kole
ReplyDeletePark
1. This microfluidic device presents a docking method to capture non-adherent cells into wells by using a receding meniscus that forces the cells to the bottom of the wells. This allows for single-cell analysis.
2. How does this method compare to the use of traps? What sort of advantages are there?
3. The limitations of this device and the advantages of this device
Erica Curtis
ReplyDeletePark
1. This cell docking method captures a small number of cells (variable depending on the size of the microwells) in wells with lateral capillary force of the receding meniscus.
2. How long do the cells continue to live without being replenished with media? During this time, how long do the cellular functions continue at the same level as cells being fed media? Is there any way that this device can be adapted in order to deliver media continuously to the cells?
3. A comparison between this method and the use of traps.
Elizabeth Lillie
ReplyDeletePark
1. This improved soft lithographic method for seeding and cell docking is a cheap and quick way to individually trap and study yeast cells.
2. Do the yeast cells attached to the surface of the well or are they simply sitting in the well and could be flushed out by media or something flowing over them?.
3. Other LOC studies using this single cell capturing method
Stephen Arndt
ReplyDeletePark
1. A passive method for the loading of cells into wells, based on evaporation
2.Define the ensemble problem
What is the rate of media metabolism by the yeast? is the relatively high percentage of yeast in traps a potential problem? would using a circular trap change the efficiency vs a square trap.
3. methods of cell loading, differences between the circular traps, and square ones
Raheel Thobhani
ReplyDeletePark
1. A soft lithographic method uses surface tension from a meniscus to trap small numbers of yeast cells into wells.
2. How long can the yeast cells remain in the wells? What happens if the yeast cells begin to divide? Will the cells remain in the wells or will they be pushed out?
3.A possible presentation could be about the yeast growth in the wells. Also, a comparison between this method and other cell trapping methods.
Elyse Sadeghi
ReplyDeletePark
1.Used receding meniscus to dock yeast cells in microfluidic wells.
2.The authors mentioned that this system could potentially be used for long-term culture of yeast cells. How is this possible since media must be replenished? They noted that flowing solution of alpha factor through the device resulted in the escape of docked cells...
3.PUA vs. PDMS
Lindsay Chatfield
ReplyDeletePark
1. A different version of soft lithography uses surface tension driven capillary flow and a receding meniscus to isolate cells into wells of MF devices.
2. Approximately how long does the process take, from the point that the cell solution is introduced to the MF device to the point that the receding meniscus reaches the inlet reservoir?
3. Comparison between the surface tension capillary driven flow/receding meniscus method and the pump-based/trap method.
Peter DelNero
ReplyDeletePark
1. The author presents the passive trapping of non-adherent cells using capillary flow and receding meniscus to isolate 1-5 cells/trap
2. How big of a problem is it that over half of the cell is outside of the media in the 1 um wells? Is there a happier medium, say 5um deep, that could isolate a small number of cells within a slightly more comfortable environment?
3. It sounds like one of the primary advantages of this device is the elimination of pumps and hardware, which might be good for cheap and portable blood assays. Perhaps a presentation on the application of this kind of device for point-of-care diagnostics.
1. Yeast cells drop into wells with capillary filling. Yeast was treated with x-alpha factor and GFP.
ReplyDelete2. How many capillary fillings does it take for the yeasts to get trap in the wells?
3. This paper is very similar to my project.
Kevin Qin
ReplyDeletePark
1. A microfluidic device that uses lateral capillary force and receding meniscus to load yeast cells into wells.
2. What is the viability of the cells after they have been loaded in the wells?
3. Ways to remedy the problems discussed in the conclusion (i.e. cells leftover in the inlet).
Tho Tran
ReplyDeletePark
1. An alternative method of soft lithography that loads and traps yeast cells through capillary force and receding meniscus.
2. Once the cells are trapped into the wells, how long can the cells survive with the given media?
3. A comparison can be made with other trapping methods. Advantages and disadvantages.
Liwei Jiang
ReplyDeletePark
1. In the authors' microfluidic device, yeast cells can be loaded such that they are isolated in PDMS microwells. Excess cells are disposed of by evaporating their media.
2. Why is evaporation allowed only at the outlet and not at both the inlet and the outlet?
3. Are there possible difficulties with this device?
Will Matloff
ReplyDeletePark
1. A receding meniscus caused by evaporation provides a downward force that can be used to trap cells into wells in a microfluidic device.
2. Device is limited by its operation. Fluid is not able to flow over cells, so main experimentation must be done beforehand. So, the device is mainly a visualization tool.
3. A presentation could explore when this device might possibly be favorable over other cell trapping devices.
Parker Gould
ReplyDeletePark
1. A device was created for the passive capture of yeast cells.
2. As with last time, is ceiling sag/collapse a possible issue here?
3. Comparison to nanophysiometer
Liwei Jiang
ReplyDeleteI thought of a better question: Wouldn't it be easier if we just have a open facing PDMS with a bunch of microwells on it? I think you can just load a bunch of cells on top, let some cells sink into those wells, and wipe away the excess cells and media with a Kimwipe.
James Irving
ReplyDeletePark
1.)The receding meniscus method does not require an external pump to provide pressure, and it allows you to trap many cells into each microwell.
2.)Why would you want to capture many cells into large wells as opposed to capturing them in traps?
3.)Compare/contrast this method of trapping with other methods.
Chaitanya Allamneni
ReplyDeletePark
1. A method of passive trapping of yeast cells was presented in which a device was fabricated that uses capillary flow and a receding meniscus to trap the cells within wells.
2. With is the stimulation response in passive vs. active flow. That is, what effect would pumping the cells into traps have versus this passive method?
3. A study on flow rates and their effects on yeast cells. Also, do the cells adhere to the wells, though not to the channels?
Brian Akselrad
ReplyDeletePark
1. Cells can be docked in wells in a micro fluidic device by a receding meniscus.
2. Do the cell ever escape from the wells during the evaporation process?
Can different solutions be added to the MF device to observe the yeast cells response, without washing the cells out of the wells?
3. Comparison of well width/length vs. depth.
Replacing media to continue cell growth.
Joe Scherrer
ReplyDeletePark
1. A receding meniscus provides a passive way to load yeast cells into wells.
2. Besides the advantage of not requiring a pump, what applications would a device like this have? Since media cannot be streamed steadily through the device, it would have major use limitations.
3. Application of the device in culturing yeast.
So through capillary action, yeast cells are isolated within cells in a microfluidic device for further study. The meniscus of the receding fluid either pushes the cells into a capture cell or sent back to the inlet. My questions are:
ReplyDelete1) Is it possible to maintain a constant, moist environment for the yeast without the use of pumps while still allowing for the capillary action?
2) Can this experiment be redone with the same batch of cells but adding more fluid?
3) How again were the yeast cells dyed?
Alan Herrera
ReplyDeletePark
1. Cell that would otherwise be difficult to capture because they are non-adherent or highly mobile, can be trapped with wells that use the force of the receding meniscus to trap the cells and media in the wells.
2. What is the force with which the receding meniscus pulls on the cells? Is it not enough to also move the cells out of the wells?
3. If this well concept is applied and the wells are made deeper, then channels could be connected to individual wells and known volumes of certain drugs, chemicals, dyes, etc. can be added to individual cells in case one needs to do preliminary tests with small samples of cells or simultaneous experimentation of multiple variable (in different trials).
Rosie Korman
ReplyDeletePark
1. Yeast cells can be docked into a microfluidic device by using surface tension driven flow.
2. What are the alterations to the protocol that need to be made in order to load adherent cells in this manner?
3. Loading adherent cells in this manner.