Journal Club
February 4th, 2010
Quantitative Metabolome Profiling of Colon and Stomach Cancer Microenvironment by Capillary Electrophoresis Time-of-Flight Mass Spectrometry
http://cancerres.aacrjournals.org/cgi/reprint/69/11/4918
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Patrick Diggins
ReplyDeleteHirayama
1. Cancer cells have an advantage in growth because they exploit their metabolism can maintain large levels of principal molecules even in conditions of limited resources.
2. Why would cancer cells be better at different metabolisms than their normal counterparts?
3. Different metabolic processes
4. Do the cells near cancer cells die because of the limited resources?
Rachel Harvey
ReplyDeleteHirayama
1. Cancerous cells rely on different methods than normal tissues for growth, due to a lack of exposure to the same resources.
2. Is autophagy as essential to growth in all types of cancer or only pancreatic and colon cancer?
3. Amino Acid concentrations in different cancerous tumors
4. -
Liwei Jiang
ReplyDeleteHirayama
1. Learned: CE-TOFMS was employed to reveal the particular metabolite characteristics of tumor cells.
2. Pressing ?: What is autophagy, and how is it important for tumor cell survival?
3. Presentation: How CE-TOFMS works; a table summarizing the findings of this paper.
4. Thoughts: I would be interested in seeing a comparison of the major MS modalities used today.
Elizabeth
ReplyDeleteHirayama
1. Capillary Electrophoresis Time-of-Flight Mass Spec can be used to process cancerous cell metabollisms
2. What is qutophagic degredation of proteins?
3. A similar paper using this type for mass spec for other diseases
1. “Metabolic adaptation” has enabled cancer cells to proliferate in a hypoxic environment with limited resources.
ReplyDelete2. Why is there evidence of aerobic respiration in both cancerous and non-cancerous stomach tissue? Are these cancer cells in a less hypoxic environment?
3. A paper using this method to analyze intermediates of fumarate respiration in order to confirm this method of respiration as the reason for high levels of fumarate and succinate in cancerous tissues.
Amy Ostrowski
ReplyDeleteHirayama
1. CE-TOFMS was used to determine that the glucose concentration in tumor cells is much lower than previously thought but levels of most amino acids were higher in cancer cells.
2. How does capillary electrophoresis mass spec work?
3. Cell's ability to use amino acids as energy sources.
Ayeeshik Kole
ReplyDeleteHirayama
1. CE-TOFMS is critical in determining cancer metabolomics for organ specific microenvironments.
2. What is the CE-TOFMS setup look like?
3. Coupling this technology with other MS techniques