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Friday, April 16, 2010

JC 4/20

Sethu et al.

Microfluidic isolation of leukocytes from whole blood for phenotype and gene expression analysis.


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8 comments:

  1. AnonymousApril 19, 2010 at 10:03 AM

    1. Leukocyte enrichment by erythrocyte lysis with deionized water- I didn't know water could be used to lyse cells.
    2. How did they fabricate the herringbone ridges on the substrate?
    3. microfluidic cell isolation techniques
    4. This experiment removes erythrocytes from the samples, but won't there still be lots of other cell types?

    ReplyDelete
  2. Tang DhummakuptApril 19, 2010 at 11:34 AM

    Tang Dhummakupt
    Sethu

    1. A microfluidic device has been made to lyse RBC with DI water while leaving leukocytes generally intact.
    2. Why are leukocytes more resistant to lysis than RBC?
    3. Procedures for purifying leukocytes, pros/cons

    ReplyDelete
  3. rachel harveyApril 19, 2010 at 1:05 PM

    Rachel Harvey
    Sethu
    1. They made a microfluidic that can lyse RBCs with water so leukocytes are isolated.
    2. What are reticulocytes?
    3. Methods of leukocyte isolation
    4. It is very interesting that RBCs can be lysed with water and not leukocytes.

    ReplyDelete
  4. funny brainApril 19, 2010 at 5:03 PM

    Niki Arinze
    Sethu
    1. A microfluidic device to isolate leukocytes by lysiing ethryocytes wil DI water
    2. Is the erythrocyte lysis due to osmotic gradients? Why would you treat the silicon wafer with oxygen plasma prior to coating with SU-8?
    3. the uses of leukocyte profiling

    ReplyDelete
  5. Liwei JiangApril 19, 2010 at 7:10 PM

    Liwei Jiang
    Sethu

    1. A microfluidic device accomplishes leukocyte isolation by lysing erythrocytes with deionized water.
    2. Where does the PBS leave the device?
    3. Classification of blood cells
    4. The phrase "leukocyte enrichment" sounds scary.

    ReplyDelete
  6. Stephen ArndtApril 20, 2010 at 9:10 AM

    Arndt
    Sethu
    1. ditto, a mf device can be used to selectively lyse erythrocytes but not leukocytes
    2. why are leukocytes more resistant than RBCs?
    3. Microfluidic translations of macro technologies

    ReplyDelete
  7. HussainApril 20, 2010 at 9:54 AM

    Jinnah
    Sethu

    1. MF device uses dH20 to isolate leukocytes by selectively lysing erythrocytes.
    2. What's the mechanism of action here?
    3. SyBBURE applications

    ReplyDelete
  8. Chaitanya AllamneniApril 20, 2010 at 10:19 AM

    Chaitanya Allamneni
    Sethu

    1. A microfluidic device can be used to lyse red blood cells with water while leaving the leukocytes unharmed.
    2. How are the leukocytes not damaged or activated by water?
    3. The feasibility of the clinical use to isolate leukocytes within microfluidic devices

    ReplyDelete

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