A Microfluidic Pipette for Single-Cell Pharmacology
Alar Ainla
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Lindsay and Raheel
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
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Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
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An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Jason Kappa
ReplyDeleteAinla
1. This paper discusses the design of a pipette that creates a recirculation zone to deliver particular media to single adherent cells, allowing the formation of a dose response curve without cross contamination.
2. What exactly do they mean by recirculation? Is it just the creation of a zone of flow where the same media is being recycled, only affecting a small group of cells? Also, what makes Chinese hamster ovary cells a good choice for this application?
3. A presentation could cover the application of single cell drug delivery. Also, some other biological applications of this pipette
Trevan Locke
ReplyDeleteAinla
1. The device in this paper is a microfluidic PDMS pipette that allows media to be delivered to areas as small as a single cell without contaminating an entire sample.
2. I guess I'm a little confused on what they mean by recirculation too. Also, can you explains how they guarantee that the media in the pipette doesn't spread farther than its meant too? I didn't really get the explanation on that.
3. Other applications of the device.
4. Seems like this could be a pretty useful device.
Laura McBride
ReplyDelete1. The paper discusses the design and features of a microfluidic pipette used to deliver complex compounds to small areas without contamination.
2. I am with Trevan and Jason, to what specifically does recirculation refer? What other proof is there to suggest that the media only effects the area to which it is applied?
3. Discussion about the other possible applications of this device.
1. Can make microfluidic PDMS pipette to make highly precise fluid changes around single cells via flow recirculation
ReplyDelete2. How do you fabricate a device to have smoothly varying thicknesses (as shown in Figure S1 c in supporing info)?
3. Compare this microfluidic pipette method of analysis to the other methods mentioned in the article (like chemistrode, microfluidic droplet dispenser for MALDI sampling, flow photolysis, etc). Do you agree with the authors' conclusion that their microfluidic pipette is better?
Samat Kabani
ReplyDeleteAinla
1. A microfluidic pipette was created to deliver solution on a single cell level without exposing the solution to other cells nearby
2. Could a vacuum be attached to the device to possibly get a single cell attached to the pipette?
3. Glass Pipettes versus Microfluidic Pipette
Tang Dhummakupt
ReplyDeleteAinla
1. A microfluidic pipette has been fabricated that exposes single cells to a solution that can be quickly switched.
2. How do the solenoid valves and switches work?
3. How a patch clamp works and how it's used.
Alex Garza
ReplyDeleteAinla
1. microfluidic pipette that can expose a pharmacological solution to only single cells without exposing the rest.
2. if you expose it to live cells, would they not move or grow and therefore spread causing exposure to other cells. what would you use it for as exposing a single cell does not mimic a biological environment?
3. comparison to other pipettes and to other methods that claim to expose only a single cell.
1. This article describes a method of in vitro "targeted drug delivery". A recirculation system within a PDMS micropipette is utilized, creating an adjustable rotating droplet at the tip which allows for manual delivery to specific cells.
ReplyDelete2. Could this system be used to isolate cells in a mixture based on phenotypic differences to study cytokines and cell communication? Are there issues with sterilization, or does the recirculation cause the effects of exposure to become negligible?
3. Other PDMS environments that can build on this concept.
Arndt
ReplyDeleteAinla
1. A swiss army knife version of the normal micropipette was made with soft lithography.
2. with Tang,How do the solenoid valves and switches work? Also how did they manipulate the pipette again?
3. basic lab tools that could see microfluidic versions
Amy Ostrowski
ReplyDeleteAinla
1. A microfluidic pipette was fabricated that uses flow recirculation to deliver solutions to a single cell without contaminating the surrounding environment.
2. How long would an average exposure experiment last?
3. Provide potential uses for such a pipette and an overview on other pipettes currently being used.
Elyse Sadeghi
ReplyDeleteAinla
1.A microfluidic pipette was constructed out of PDMS to deliver solutions to single cells
2.How did they test that they were actually recirculating the media as they wanted?
3.Advantages of this pipette over microfluidic glass pipettes
Harvey
ReplyDeleteAinla
1. A PDMS micropipette was made to expose single cells to circulating solutions.
2. Having a vacuum sucking up the solution won't have any effect on the cell that is being exposed? Wouldn't the cell be sucked towards the micropipette too?
3. Exposure of single cells to solution by varying methods
Elizabeth Lillie
ReplyDeleteAinla
1. A micropipette was fabricated from PDMS that allows for flow recirculation and single cell analysis.
2. What is the benefit of using this micropipette over the other LOC, single cell analysis asseys?
3. Other studies using this micropipette.
Kevin Qin
ReplyDeleteAinla
1. The development of a PDMS micropipette that can expose single cells to solution via recirculation.
2.How does recirculation and the switches work? How is the recirculation volumed calculated by the channel outlets and flow rates?
3.PDMS micropipette assays vs. regular micropipette assays.
Lauren Kolski
ReplyDeleteAinla
1. A micropipette was fabricated out of PDMS and allowed for pharmacological screening of intact adherent cells without affecting the surrounding cells
2.What exactly is the "circulating liquid tip?"
3. Advantages of this micropipette over the usual glass micropipette. What are the benefits of stimulating only one cell vs. stimulating all cells and then just performing many different experiments?
Liwei Jiang
ReplyDeleteAinla
1. Learned: a microfluidics pipette using a recirculating tip is used for single cell assays.
2. Pressing ?: (1) What is "localized superfusion"? (2) How does recirculation avoid contamination?
3. Presentation: I think the best thing to do is to explain "recirculation" in some detail.
4. Thoughts: I would imagine that this pipette is not intended for chemotaxis assays, for example, where the pipetted fluid is expected to diffuse out.
Katherine Roth
ReplyDeleteAinla
1. A microfluidic pipette made from PDMS is used to introduce and regulate specific solutions around single cells by flow recirculation.
2. Can you explain the concept of recirculation and the relationship between the open volume and recirculation zone?
3. Other methods of single cell exposure and other applications of the microfluidic pipette.
Kevin Roman
ReplyDeleteAinla
1. A PDMS microfluidic pipette that uses recirculating tip for single cell assays.
2. How does the recirculation avoid contamination?
3.Compare contamination rate to other single cell methods.
James Irving
ReplyDeleteAinla
1. The paper reveals a microfluidic pipette that uses 3 channels in order to selectively expose single cells to solutions without contamination. Contamination is avoided by recirculating the solutions into the device.
2. How do the solenoid valves work?
3. A presentation that compares this pipette to other pipettes that are used primarily to avoid contamination while selectively exposing single cells.
1. A microfluidic pipette was created to deliver solution on a single cell level without exposing the solution to other cells nearby by using a method of recirculation.
ReplyDelete2. Explain recirculation,and its effectiveness.
3. various experiments that make this method advantageous
Erik Werner
ReplyDeleteAinla
1. A MF device designed to act like a micropipette, creating a versatile tool for single cell assays.
2. How do the flow switches work?
3. A presentation on the different applications this device can be modified to accommodate.
Chaianya Allamneni
ReplyDeleteAinla
1. A micropipete was designed out of PDMS that can selectively provide certain single cells with specific fluids through flow recirculation
2. More details into the principles behind recirculation, how long can fluids be recirculated in device before they are contaminated
3. What is the advantage of selectively altering flow of fluids into a microfluidic device, how precise is the micropipete, why not instead use several devices, each filled with diff. fluids
Ayeeshik Kole
ReplyDeleteAinla
1. The MF pipette can deliver complex, uncontaminated, mixed fluids in volumes to control the chemical environment of a single cell.
2. How exactly do they control this pipette (explain the schematic)?
3. Possibilities of incorporating this technology with trap devices
Will Matloff
ReplyDeleteAinla
1. A multi-functional microfluidic version of a micropipette was created that uses tip circulation to deliver a solution confined to a single cell.
2. How do they create good recirculation without creating inward flow that would pull cells into the device?
3. How this delivery technique might be used in a device such as a cell trap.
Brian Akselrad
ReplyDeleteAinla
1. A microfluidic device can be used to make a micropipette capable of delivering solutions to singles cell, using recirculation methods, and is better than glass micropipettes.
2. I would like to understand the recirculation and flow-switching better.
3. Simple study of MF pipette vs. glass pipette.
Rosie Korman
ReplyDeleteAinla
1. A microfluidic pipette that is able to control the concentration of various substances around a single cell has been developed.
2. What is multiplexing?
Is diffusion through the PDMS a problem?
3. Possible therapeutic applications of this device
Ainla
ReplyDelete1. A MF pipette that can deliver defined and tunable amounts of fluid has been fabricated and tested.
2. The whole cell patch clamp method?
3. Better than a pipette on a micromanipulator?