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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
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Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Lauren Kolski
ReplyDeleteIkeda
1. They formulated a microfluidic device to lyse single cells using either the force generated by electroosmotic flow or an alternating electric field.
2. How does the "sputtering" process work?
3. The benefits of single-cell analysis.
4. How does this device an improvement on the MEMS technology?
Rosie Korman
ReplyDeleteIkeda
1. Ikeda et al. have developed a MF device that can lyse cells in two different ways - by pinching the cells and/or by creating an electric field around them.
2. Why zucchini protoplast cells?
How does electroporation work?
3. Chemical methods for lysing cells.
Trevan Locke
ReplyDeleteIkeda
1. They developed a device that can lyse single cells using pinching or an electric field.
2. Why did they use zucchini protoplast cells? Are there limitations to the types of cells this device can work with?
3. Why lysing is important/useful.
Peter DelNero
ReplyDeleteIkeda
1. Device for capturing and lysing single cells with a pinched-channel and parallel electrodes.
2. How long does it take to capture and lyse a cell? How quickly could the device be re-loaded?
3. electro-osmotic flow- how it works and devices that use it.
4. This device is like a cellular electric-chair. I'm picturing the stereotypical mad scientist...
Elizabeth
ReplyDeleteIkeda
1. This group developed a single cell lysis device that tests both lysis methods using both mechanical and electrical techniques.
2. Does lysis via electroporation disrupt the cell membrane or does it also denature proteins and other organelles within the cell?
3. Compare and contrast this and PONG
Brian Akselrad
ReplyDeleteIkeda
1. A microfluidic device was designed to lyse single cells using a pinched channel and electrodes.
2. After the cells lyse, what happens to the intracellular material? And can you explain how the device knows how much electricity to use based on cell type/size?
3. How does this device compare to other methods; pros and cons.
Alex Garza
ReplyDeleteIkeda
1. They made a MF device that can lyse cells by pinching or with electrodes.
2. Does it only lyse the cell membrane or can it lyse other parts of the cell as well?
3. compare with chemical ways of cell lysing
This comment has been removed by the author.
ReplyDeleteLaura
ReplyDeleteIkeda
1. The article discusses the use of a microfluidic device designed to lyse cells by pinching or using an alternating electric field.
2. Does capturing the cells take a significant amount of time? Why are zucchini cells use? Is this an indication of the devices limitations?
3. Compare this device to others. Why lyse?
Rachel Harvey
ReplyDeleteIkeda
1. A MF device was made that can lyse cells by using parallel electrodes to induce an electric field or pinch cells.
2. Why zucchini cells? What does protoplast mean?
3. Methods of single cell lysis
4. Would all cells lyse at a similar voltage/ strength of electric field? Is there a large gap between adenocarcinoma cells and healthy colon cells that would cause differences in the voltage necessary to lyse?
Lindsay Chatfield
ReplyDeleteIkeda
1. A microfluidic device "has been fabricated to enable easier single-cell capture and lysis." It uses one of two methods - either the through force generated by EOF or alternating voltage.
2. The authors claim that "the electrical lysis method is selective towards the plasma membrane while leaving the organelle membrane undamaged." How does this work? What is the difference in the membrane compositions that accounts for this?
3. Why do we want to lyse a single cell?
Ayeeshik Kole
ReplyDeleteIkeda
1. The authors created a MF device that allows for single-cell lysis using mechanical stress and/or applied voltage.
2. How does transfection of cells uses electroporation to introduce DNA to cells without lysing them?
3. Importance of intracellular content analysis
Will Matloff
ReplyDeleteIkeda
1. A microfluidic device was developed that can capture and lyse cells using both a pinched channel structure and two pairs of electrodes.
2. What exactly about the alternating voltage causes the captured cells to break?
3. A presentation could be given on the current state of the art of cell lysing.
Chaitanya Allamneni
ReplyDeleteIkeda
1. A microfluidic device was designed that can lyse cells either by pinching them or "shocking" them via an electric field generated through parallel electrodes.
2. How do different cell types vary in their maximum "shoc" field before lysing. That is, how might a cardimoyocyte respond differently to a certain electric field than another cell type.
3. Advantages of electrical vs. chemical lysing
This experiment involved the lysing of single cells either by the indirect application of electricity (via EOF) or the direct application of it. My questions are.
ReplyDelete1) What is the smallest cell type, this device can be used on? What is the largest?
2) If the cell in question is stuck to other cells via some sort of binding action, can this device be used to actively separate the cells?
3) Can this device be portable or does it need to be plugged in?
Katherine Roth
ReplyDeleteIkeda
1. A microfluidic device designed to lyse cells by either pinching them off or by applying an electric field.
2. Why is the plasma membrane favored? And is there a known correlation between cell size/shape and the amount of "shoc" required before lysing?
3. Studies where cell lysing has been an important factor.
Jake Brady
ReplyDeleteIkeda
1.) a microfluidic method of lysing cells by utilizing either physical force of pinching a cell by way of fluid flow generated by EOF or by introducing an electrical field.
2.) How does the current cause the cell to burst, and why only the plasma membrane and not organelle?
3.) a presentation on the other types of cells that can by lysed and why it would be useful.
Erik Werner
ReplyDeleteIkeda
1. A MF device was designed to lyse cells by pinching, and electric fields were used both to direct the cells into pinch structures and to lyse the cells.
2. How hard is a zucchini cell's plasma membrane compared to an animal cell. Why zucchini? Are vegetables even important for this type of research?
3. Compare/contract with other methods for lysing cells.
Samat Kabani
ReplyDeleteIkeda
1. They created a microfluidic device that uses electric fields to capture and lyse single cells in order to extract intracellular components without damage.
2. Are there any specific voltages/frequencies for lysing T cells?
3. Single-cell analysis
Joe Scherrer
ReplyDeleteIkeda
1. Electrodes embedded in a microfluidic device were used to manipulate and lyse cells using electroosmotic flow.
2. Is there a correlation between cell size and lysing potential?
3. Discuss the speed of lysing multiple cells.