Hi Everyone,
Here is this week's article. A pdf can be found here.
Thanks,
Laura and Will
Subscribe to:
Post Comments (Atom)
skip to main |
skip to sidebar
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
JOURNAL CLUB BLOG
Posts
Welcome to the SyBBURE Journal Club
Rules:
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
Search This Blog
Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Tang Dhummakupt
ReplyDeleteGervais
1. A microfluidic device can be used in a 1-step assay immunoassay in point-of-care diagnostics.
2. What was the point of the 3rd set-up where they had perpendicular dABs deposition channel? Why did they want to trap air bubbles?
3. How conventional immunoassays work and what are they used for.
Trevan Locke
ReplyDeleteGervais
1. They developed a one step immunoassay device for use in point-of-care diagnostics
2. How do the flow resistors resist flow?
3. Point-of-car diagnostics on the microfludic level.
Elizabeth Lillie
ReplyDeleteGervais
1. 1 step immunoassays can be performed in microfluidic devices and applied to point-of-care diagnostics.
2. Are the cAbs and dAbs targeting any general antibody in the blood or are they more specific (if so what are they targeting)?
3. Other papers regarding the use of microfluidics in point-of-care diagnostics
1. Immunodiagnotics on a chip.
ReplyDelete2. In figure 2d. the article said that "The rounded hexagonal posts are used to define a reproducible filling behaviour that minimizes the
formation of air bubbles",can you explain how it works?
3. Any papers that deal with microfluidics.
Jason Kappa
ReplyDeleteGervais
1. This paper discusses the design of a single step immunoassay that can translate microfluidics to the clinic, requiring only a patient sample and a fluorescent microscope to run a test.
2. What exactly are the antibodies targeting? Are they generalized antibodies to demonstrate a concept or was this design targeted for a specific cell type or antigen?
3. A presentation could be given on potential diagnostic targets of a one step chip like this one.
Peter DelNero
ReplyDeleteGervais
1. Lab-on-chip device uses capillary forces to run one-step immunodiagnostics- perfect for POC applications.
2. How can you calculate capillary pressure in the different regions of the device? How does the "capillary pump" create pressure?
3. It seems like there should be an easier way to avoid air bubbles in this device than the series of delay valves. Is this a common feature for loading POC devices?
4. This device seems ideal for applications in low-resource areas like developing countries or military, if the antibodies could be selected for specific infections. The only difficult equipment is the microscope.
Liwei Jiang
ReplyDeleteGervais
1. A microfluidic device that does immunoassays with on-chip reagents
2. How do delay valves work?
3. Other (earlier) point-of-care immunodiagnostic devices
4. We have seen a lot of these papers, but have any of these devices actually gained widespread usage?
Jinnah
ReplyDeleteGervais
1. The authors present a capillary-based microfluidic platform for quick single-step immunoassays (this version senses CRP) using very small amounts of reagents.
2. Any limitations on the types of molecules that can be detected with this device? (I suppose it depends on the reagents you introduce)
3. Other complications with this device design other than the bubble issue.
Rachel Harvey
ReplyDeleteGervais
1. One step immunoassays can be done in microfluidics and applied to point-of-care diagnostics.
2. How do the pumps work? What antigen is the antibody specific for?
3. Point-of-care diagnostic microfluidics
This project is to produce a better chip designed to analyze human blood. My questions are:
ReplyDelete1) What is the shelf life of this device? How are they keeping the passages in the PDMS hydrophilic?
2) Is there a problem with deposition of the solid components of blood in the passages? If so, are there any measures to prevent this?
3) Would external temperature affect its effectiveness? Could this device be used everywhere?
This comment has been removed by the author.
ReplyDeleteBrian Akselrad
ReplyDeleteGervais
1. Point-of-care diagnostics using a microfluidic device capable of one-step immunodiagnostics.
2. What exactly do the dAbs and cAbs antibodies attach to? They must target something specific.
3. One-step point-of-care immunodiagnostics seem so great, why don't we see more of them actually being used, or do I just not realize that so many devices fall under that category?
Raheel Thobhani
ReplyDeleteGervais
1.Capillary driven microfluidics are used for point-of-care diagnostics.
2.How do the valves and pumps work?
3.Practical uses for these devices.
Erik Werner
ReplyDeleteGervais
1. A microfluidic device was developed to perform one-step immunoassays for point of care diagnostics
2. Will capillary action pull the fluid through the entire device? Otherwise, how does the capillary pump placed at the end of the device work?
3. How close are MF POC devices to being ready for clinical use? Any examples of existing devices?
Katherine Roth
ReplyDeleteGervais
1. A microfluidic device used for one-step immunoassays for point-of-care diagnostics.
2. Please explain how the design for minimizing air bubbles works.
3. What are the projects being done here at SyBBURE that relate to this?
Ayeeshik Kole
ReplyDeleteGervais
1. A microfluidic device with integrated sandwich immunoassays provides one-step point-of-care diagnostics
2. Is there an assay that can tell us what sort of chemotherapy to administer for cancer patients?
3. Capillary pump mechanisms
Chaitanya Allamneni
ReplyDeleteGervais
1. A microfluidic device can use 1-step immunoassays for point of care studies through capillary force.
2. They discussed CRP, but what are other cardiac markers that indicate if one is at risk for myocardial infarction?
3. Also, explain how capillary force causes flow of substances through device.
This comment has been removed by the author.
ReplyDeleteGervais
ReplyDelete1. Single step immunoassay for point of care studies.
2. Do you think we could use an Autodrop printer like that to do shrinky dinks?
3. More diagnostic test-type MF devices
Jake Brady
ReplyDeleteGervais
1.) on chip immunoassay using single step capillary action for point of care use.
2.) elaborate on the air-bubble system and how it works
3.) other point of care devices - if any - that perform immunoassays and explains how conventional immunoassays work.
Samat Kabani
ReplyDeleteGervais
1. A microfluidic device was made to carry out an immunoassay with one step.
2. How quickly can they be made?
3. One-point Diagnostics Immunoassays