Here's this week's article: http://www.bme.ucdavis.edu/simonlab/pdf/Vascular%20mimetics%20based%20on%20microfluidics.pdf
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
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Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Lauren Kolski
ReplyDeleteSchaff
1. They created a microfluidic device for the study the kinetics of leukocyte capture, rolling velocity, and deceleration to arrest in response to addition of chemokine. This device improves on many of the shortcomings of the parallel plate flow chamber device currently in use.
2. How does the chemokine exposure and resulting increase in adhesion occur in the mammalian body? What is the biological importance of this process?
3. Before delving into answering the questions, give a brief yet informative overview of the biology discussed in this paper; talk about the inflammatory response, role of leukocytes, the idea of "rolling neutrophils", etc
4. Very clever using vacuum sealing rather than plasma bonding. Do either of you plan on doing something like this in your projects?
Rosie Korman
ReplyDeleteSchaff
1. The authors created a MF device that allows imaging of leukocyte activation and adhesion to an endothelial layer. Since the MF device uses a smaller amount of reagents, leukocyte activation/adhesion studies can be performed for a longer period of time.
2. Why does the syringe pump use negative rather than positive pressure to drive flow through the device?
Why is shear stress higher in the inner flow branches?
3. Other applications of the vacuum network design.
Trevan Locke
ReplyDeleteScaff
1. They created a device for the study of leukocytes that can be bonded to a cell monolayer. It represents a significant improvement over previous devices
2. Can you explain more about what they were hoping to see using this device?
3. Application of the vacuum sealing idea to Sybbure projects.
Elizabeth Lillie
ReplyDeleteScaff
1. A microfluidic device was developed to mimic venules and study the inflammatory response on neutrophils and the use of chemokines in rolling and stopping the cells.
2. Other than experimental benefits over the previously used PPFC, what new information can be gathered from this device?
3. A paper on this device testing their theory on the deactivation of rolling by Mac-1
Jason Kappa
ReplyDeleteScaff
1. This papers discusses the development of a microfluidic device that allows for the observation and imaging of leukocyte adhesion and interaction with an endothelial cell coating. This device is an improvement over previous parallel plate models because it has high throughput while still maintaining compatibility with cell substrates.
2. How accurate is the simplified equation for shear that they used? In order for enough terms to drop out, they had to have assumed that there was no flow in the vertical axis. Is this a safe assumption, considering that the channels were much taller than the size of the cells?
3.A presentation could highlight the fundamental assumptions of their fluid flow shear model.
Samat Kabani
ReplyDeleteScaff
1. The authors created a microfluidic device that allows for the imaging of leukocytes on a endothelial monolayer for observance of adhesion and rolling.
2. Would varying the flow rate affect the adhesion of the leukocytes?
3. Leukocyte Activation in High Flow Areas
Lindsay Chatfield
ReplyDeleteScaff
1. A microfluidic device was designed using vacuum sealing to image leukocyte-endothelial interactions at a defined shear force. Specifically, neutrophil adhesion to an endothelial monolayer was monitored in response to the introduction of chemokine (IL-8).
2. Have biological substrates other than an endothelial monolayer been tried?
3. Advantages and disadvantages of positive and negative pressure pumping.
Liwei Jiang
ReplyDeleteSchaff
1. A vacuum sealed microfluidic device was used to study surface interactions of leukocytes
2. What is the "rolling" behavior of leukocyte-endothelial interactions?
3. Explain why the rolling velocity is approximately uniform across the channel even though the sheer stress varies.
Rachel Harvey
ReplyDeleteSchaff
1. Neutrophil extravasation was modeled and imaged in a microfluidic device with an endothelial monolayer.
2. What minimum amount of IL-8 is required for Neutrophils to roll and adhere?
3. Extravasation in Microfluidic Device
Peter DelNero
ReplyDeleteSchaff
1. A microfluidic device was used to mimic the vascular environment and study leukocyte response to defined concentrations of signal molecules.
2. Are there any other microfluidic devices that seal to well-plates? If so, what did they use to create the seal instead of a vacuum, and why did this not work for Schaff's device?
3. Biomimetic microfluidics- examples of other devices that have been made to model in vitro conditions
4. I thought it was cool that they have an effective method of modeling the vascular environment by coupling a microchannel to a well-plate. One of the graduate students in the Swartz lab is doing the same thing but for the reverse process: he is trying to make a device to show cancer cells migrating through tissue towards draining vessels.
Shaun Kahler
ReplyDeleteSchaff
1. The authors created a microfluidic device bonded with vacuum pressure to monitor how IL-8 affects the binding of neutrophils to a substrate.
2. What is the limit to which vacuum binding is sufficient to hold a device down? Would it work for a device with finer details like the T cell trap?
3. This experiment with multiple substrates of varying density, % fluid, etc. (i.e. bone, organ tissue, muscle, etc.)
James Irving
ReplyDeleteSchaff
1. The journal describes a microfluidic device on an endothelial layer, used to study the dynamics of leukocytes with that layer.
2. Could you elaborate on the advantage of using negative pressure on the outlet over using positive pressure at the inlet?
3. Give background on parallel plate flow chamber devices.
Hussain Jinnah
ReplyDeleteScaff
1. The authors designed a MF system (device vacuum bonded to culture plate) for studying & imaging the leukocyte-endothelial inflammatory response.
2. What are the conventional methods of studying this inflammatory response?
3. plasma vs vacuum bonding
Erik Werner
ReplyDeleteScaff
1. A MF device was created using a vacuum as a novel method of PDMS bonding, and Neutrophil extravasation was modeled and studied in this system.
2. What are the limits of the vacuum/negative pressure system? How thin can the PDMS be and how much force can be applied this way before it deforms?
3. How this technology could enhance the study of the inflammatory system, perhaps by adding things to that reservoir.
4. How hard would this be to do in our labs/has anyone here tried it before?
This group designed a superior device designed to study the inflammatory response pathways.
ReplyDeleteMy questions are:
1) How exactly does the vacuum system work?
2) Can this design be used elsewhere?
Chaitanya Allamneni
ReplyDeleteSchaff
1. A microfluidic device was designed that was directly vacuum bonded to a 6 well plate, and particularly useful because real time cell culture could be examined within a microfluidic platform. Specifically, the leukocyte inflammatory response was studied.
2. Teach me more about vacuum bonding and its advantages for cell culture studies. Sounds like it could be useful for those working with cells in SyBBURE.
3. Inflammatory response is a broad, non-specific response ; what other releases could be studied in the device
Raheel Thobhani
ReplyDeleteSchaff
1. A microfluidic device was used for imaging leukocyte interaction with a biological substrate.
2.What other biological substances can be used?
3. A comparison between plasma and vacuum bonding.
Will Matloff
ReplyDeleteSchaff
1. A microfluidic device was created to study leukocyte interaction with a biological substrate.
2. Can the device design be explained in further detail, particularly the connection of the device to the monolayer?
3. A presentation could be given on biological readouts of inflammation and the significance of this device.
Ayeeshik Kole
ReplyDeleteScaff
1. The microfluidic device can help study the leukocyte rolling cascade for detailed kinetics.
2. How can the mechanics of this device be extended beyond studying this inflammatory response?
3. Importance of biomimetics
Erica Curtis
ReplyDeleteSchaff
1. They developed a MF device that can be used to observe neutrophil "rolling" and adhesion on a endothelial monolayer. This device has a higher throughput than the parallel plate flow channel.
2. In what situations is vacuum bonding preferential and/or feasible?
3. Explain the design of the parallel plate flow channel. List the improvements in the MF device and what advantage each improvement provides.
Katherine Roth
ReplyDeleteScaff
1. A microfluidic device designed for the study of leukocytes in the inflammatory response, including capture, rolling velocity, and deceleration due to chemokines.
2. Can you explain the specifics of vacuum bonding and also of the parallel plate method?
3. An overview of the design/set-up of this device.
1. They created a MF device to study leukocyte interaction with certain signal molecules. It is a significant improvement to the parallel plate flow previously used.
ReplyDelete2. I would also like to know the specifics of vacuum bonding and how exactly the parallel plate method works.
3. Other responses that can be studied aside from the inflammatory response.
Brian Akselrad
ReplyDeleteSchaff
1. A microfluidic device was created to image and study the leukocyte rolling phenomenon that occurs in response to acute inflammation.
2. Why did they do vacuum bonding instead of plasma bonding and what are the benefits to vacuum bonding.
3. I would like to see the images that this device enables researchers to take.