Here is the article for 7/9. Please post your pcr as soon as possible. Happy blogging!!!!
http://www.nature.com/nmeth/journal/v7/n5/pdf/nmeth.1452.pdf
thanks,
Bao Nguyen
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
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Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Peter DelNero
ReplyDeleteGodin
1. This article describes a novel method for monitoring the mass of individual cells over time by repeatedly measuring them with a suspended microchannel resonator.
2. Could you please explain how the pumps worked? It sounds like they had very precise control of the flow, which was interesting. Do we ever use precision pressure regulators? How do solenoid valves work?
3. Fabrication of the SMR
4. Is there someone in Sybbure trying to measure cell mass? Is it desirable to measure the mass of cells in the cell traps? How would this be possible?
Lauren Kolski
ReplyDeleteGodin
1. They created a microfluidic device containing a suspended microchannel resonator that is capable of dynamically trapping individual cells and continuously monitoring cell size and growth.
2. They state that when finding the instantaneous growth rate of the cells, the results do "not necessarily depend on knowing the position of each cell in the cell-division cycle." How would one justify this claim given that cells do not grow at a constant rate through the cell cycle?
3. Discuss the idea of dynamic trapping
Amy Ostrowski
ReplyDeleteGodin
1. A suspended microchannel resonator was used to dynamically trap a cell and take consecutive measurements of the buoyant mass to monitor the growth rate of the cell.
2. I understand how the cell is trapped but buoyant mass is dependent on volume and the density of the cell and fluid, could you explain how the frequency shift can then be simply converted to buoyant mass?
3. SyBBURE projects that could use dynamic trapping.
Trevan Locke
ReplyDeleteGodin
1. They created a device that traps cells using a suspended microchannel resonator. They used this device to measure cell growth rates.
2. Are you planning on using something like this with you projects?
3. Discussing dynamic trapping and its applications
1. Through the use of a suspended microchannel resonator, we can measure buoyant mass of single cells and correlate that measure with the growth rates of cells of varying sizes.
ReplyDelete2. How can we use the SMR for studying apoptosis, as the authors suggest?
3. Explain the fluid forces that allow the SMR to measure precisely
Kevin Qin
ReplyDeleteGodin
1. A microfluidic device was created that is capable of capturing individual cells and monitoring their instantaneous growth rate via a suspended microchannel resonator.
2. Why is the device not optically accessible?
3. Dynamic trapping vs. other trapping methods.
Hussain Jinnah
ReplyDeleteGodin
1. The authors made a MF device that quantifies the growth rate of individual cells by taking repeated measurements of buoyant mass.
2. Is there a limit on the type of cells that can be massed using this device design?
3. Brief overview of the SMR layout.
Stephen Arndt
ReplyDeleteGodin
1. these bro's made a MF device capable of localizing a cell, and based on its bouyant mass, the size is calculated.
2. I may have missed it but this assumes a constant shape? Follow up, how does it deal with microtubule reshaping, e.i. a biconcave disc vs a sphere could have the same volume but much different surface area, and any remodeling could cause a difference in buoyant force, is this considered noise? Does the resonance get the cell to maintain shape?
3. comprehensive outline of cellular remodeling and the effect on noise in measurements
Raheel Thobhani
ReplyDeleteGoldin
1. The authors made a microfluidic device that helps measure the buoyant mass of a cell and its growth rate.
2. What exactly is a dynamic trap? Is it just a trap created by differing flow directions? Also, can this work for all cells?
3. Discuss dynamic trapping and how the SMR uses the fluids.
Erik Werner
ReplyDeleteGodin
1. A MF device capable of measuring a cell's mass at fg resolution was used to measure the growth rates of single cells.
2. How does the feedback algorithm that reverses flow work?
3. Go over the details of the SMR. It looks like the same group published on it months before.
4. I don't get why the device isn't optically accessible. The vacuum? Volume seems pretty important to me. It must be to them too, as they spent half a page justifying it's omission at the beginning.
Elyse Sadeghi
ReplyDeleteGodin
1.They developed a microfluidic device to trap single cells and measure their buoyant mass to determine growth rate.
2.How do they know which cells are heavier vs. which cells are just about to split into two daughter cells?
3.Measuring growth rate of adherent cells
James Irving
ReplyDeleteGodin
1.The authors created a microfluidic device which dynamically traps single cells on a microcantilever that can determine the cells’ buoyant mass in order to determine the growth rate.
2.Why does the device have a difficult time trapping a cell for extended periods of time (like an entire growth cycle)?
3. Compare this to other methods for determining growth rate.
Lindsay Chatfield
ReplyDeleteGodin
1. A "dynamic fluidic control system" with a suspended microchannel resonator (SMR) was created that is capable of measuring the buoyant mass of a single cell with femtogram resolution.
2. What is the distinction between "buoyant mass" and regular "mass"? Also, how are frequency measurements converted to mass measurements?
3. Fabrication of this device. Its applications to current/future SyBBURE projects.
Liwei Jiang
ReplyDeleteGodin
1. A device with a resonating cantilever is used to measure the mass of single cells to monitor their growth.
2. Does the cantilever obey simple harmonic motion ( omega = sqrt{k/m} )? If so, how do the authors know what the spring constant k is?
3. Some growth models of different types of cells.
Ayeeshik Kole
ReplyDeleteGodin
1. The MF device can measure the growth rate of a single cell by 'instantaneously' tracking the buoyant mass using SMR technology.
2. How does the microcantilever system operate? What kind of success rate is there with fabrication of this device?
3. Comparison of results to other growth rate measurement techniques.
Alex Garza
ReplyDeleteGodin
1. Discussion of a MF device that can measure the weight of single cells.
2. Does the device only measure certain kinds of cells or does it work for all cells? Is there a conversion between buoyant mass and mass?
3. comparison of masses obtained by using different techniques.
Jason Kappa
ReplyDeleteGodin
1. This paper discusses a device that utilizes microfluidic flow dynamics to trap a cell within a suspended microchannel resonator, which can determine a cell's buoyant mass based on the disruption of a resonant frequency.
2. How exactly did they measure "instantaneous" growth rate based on buoyant mass? Was it the change in mass over a given measuring period?
3. Comparison of this cell trapping system with the PONG device discussed in an earlier journal club.
Rachel Harvey
ReplyDeleteGodin
1. Individual cells' buoyant masses were measured using SMR over time to monitor growth.
2. What are some other applications of SMR?
3. Instantaneous growth rates of different types of cells.
4. What other cell types are usable?
Godin
ReplyDelete1. A microchannel resonator was used to measure cell growth by the change in their buoyant mass.
2. How do you convert frequency shift to buoyant mass/growth rate?
3. The explanation for the outliers in Fig. 2d. seems a little shaky...
Will Matloff
ReplyDeleteGodin
1. A microfluidic device was created that can measure the growth rate and buoyant mass of a single cell using a suspended microchannel resonator.
2. Could a suspended microchannel resonator be used in a trap device to measure a trapped cell's mass and growth rate?
3. A presentation could be given on how the resonator works.
Chaitanya Allamneni
ReplyDeleteGodin
1. A microfluidic device was fabricated that can montitor cell growth through change in mass in real-time through the use of a resonating microcantilever.
2. Deeper explanation of SMR and how resonant force can be correlated with mass.
3. I actually came across the "microcantilever" as a means to measure cardiac contractility in some previous literature searches. What are other possible biological uses for the microcantilever.
This experiment involved the dynamic measurement of cell size and growth using a resonance. My questions are:
ReplyDelete1) How accurate is such a measuring method? In many parts of the paper, the number of cells present was assumed.
2) Can cells of multiple types be present? Can an experiment involving the measurement of cell growth in a heterogeneous environment be done?
Lei Qu
ReplyDeleteGodin
Journal article first author's last name
1. A cell monitoring device for cell growth and size. This device is manufactured base on the principle of resonator.
2. Do cells undergo physical damage under resonator? Will this only work for certain cells?
3. Fabrication of the device
Katherine Roth
ReplyDeleteGodin
1. A microfluidic device that uses buoyant mass to track growth rates of single cells.
2. Can this method by applied to all cell types and shapes? Please explain the microcantilever system.
3. Specifics on how the resonator works/was designed.
Brian Akselrad
ReplyDeleteGodin
1. A microfluidic device using a suspended microchannel resonator was used to monitor the buoyant mass of cells to monitor their growth.
2. I dont fully understand how this device can trap one cell at a time.
3. I would like to see the equations behind the cantilever calculations.
Laura McBride
ReplyDelete1. The method discussed in this article outlines a a novel way to monitor cell growth with a suspended microchannel resonator.
2. What additional cell types can be monitored in this channel? What other ways can SMR be used?
3. Design of the device. Explanation behind the calculations as well.
Joe Scherrer
ReplyDeleteGodin
1. Cell growth can be measured by measuring the buoyant mass of cells with a resonator.
2. Does changing components of the media change the media density enough to affect the resonator accuracy?
3. Implementation of laser tweezers to improve accuracy of device.
Samat Kabani
ReplyDeleteGodin
1. By using a suspended microchannel resonator, the authors were able to monitor cell growth based on buoyant mass.
2. How would one create a more precise mass readout with the SMR?
3. Damping of cantilever with smaller sizes