Sorry for putting this post up so late.
Monitoring Dynamic Changes in Lymph Metabolome of Fasting and Fed Rats by Electrospray Ionization-Ion Mobility Mass Spectrometry (ESI-IMMS)
Kimberly Kaplan, Prabha Dwivedi, Sean Davidson, Qing Yang, Patrick Tso, William Siems and Herbert H. Hill, Jr.
Anal. Chem., 2009, 81 (19), pp 7944–7953
Posts
Tang Dhummakupt
ReplyDeleteKaplan
1. ESI-IMMS can be used to study the change in metabolites caused by changes in the body, possibly leading to earlier diagnosis of health problems.
2. I don't understand what's going on in figure 3. What are principle component analysis score plots?
3. How an ESI-MS works.
Samat Kabani
ReplyDeleteKaplan
1. By coupling IMS and mass spectrometer, one can analyze the changes in the mesenteric lymph metabolites which can help in early detection and prevention of chronic diseases in humans.
2. In figure 3, what does the positive and negative mode indicate?
3. Applications of Metabolomics Today
Lauren Kolski
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1. IMMS is a good analytical technique, when coupled with statistical analysis, to study the changes of metabolomes in response to external stress
2. What is the significance of "positive" and "negative" mode? Do they attract the oppositely charged ions?
3. Explanation of ESI-IMMS (with diagrams!!)
Hussain Jinnah
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1. IMMS is proving to be a great technique for rapidly measuring metabolic changes in host organisms.
2. What is IMMS used for in our labs?
3. Could you explain the apparatus in more detail?
Chaitanya Allamneni
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1. ESI-IMMS is an effective method for measuring and monitoring metabolite changes. This is clinically significant in order to discover the onset of diseases such as diabetes.
2. I'm having a hard time interpreting the graphs for each metabolite component (carbohydrate, etc). Why would it matter whether the MS was in positive vs. negative mode.
3. Is metabolomics actively being pursued today? Is lab work of this kind being used in a clinical setting to diagnose such diseases as described?
Liwei Jiang
ReplyDeleteKaplan
1. Learned: IMMS is an excellent technique for detecting and distinguishing different ions in a sample.
2. Questions: (1) What are the positive and negative ion detection modes of the IMMS? Do they correspond to opposite directions of the applied electric field that accelerates ions through? (2) What are PCA score plots?
3. Presentation: Does this paper present the best technique currently available for achieving its stated goals?
4. Thoughts: Is anyone currently employing this technique as part of his or her research?
Tesniem Shinawi
ReplyDeleteKaplan
1. IMMS is an effective analytical technique for metabolic applications.
2. Could you explain positive and negative modes and their function in these experiments?
3. other emerging techniques and how IMMS compares to them.
Ayeeshik Kole
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1. ESI-IMMS is a rapid and efficient separation technique to identify a variety of chemical classes, making it extremely useful in metabolite analysis.
2. What is a PCA analysis and how is it conducted?
3. The next step with this list of dynamic metabolites, what does it tell us?
Sami Ayad
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1. IMMS is an effective method of separating metabolic changes in samples.
2. Could you guys elaborate on the advantages of using negative or positive mode and how they affect results and measurements?
3. Has this kind of measurement and experiments with how stress affects living beings been applied to human before? if so what kind of method of metabolite analysis was used?
test again
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