Systematic investigation of protein phase behavior with a microfluidic formulator
Carl L. Hansen, Morten O. A. Sommer, and Stephen R. Quake*
http://www.pnas.org/content/101/40/14431
Presenters:
Ayeeshik Kole
Lauren Kolski
Please post by 8 PM Thursday
Posts
Niki Arinze
ReplyDeleteHansen
1. Microfluidic formaulator can be used to find solubility characteristics of proteins to improve crystallization methods
2. Why did they incubate under paraffin oil for the batch crystallization trials? How is phase behavior usually determinerd? What are virial coefficients?
3. How to go from a crystallized protein to knowing its structure
4. I wonder if the formulations found in this method are easily translatable to larger scale precipitation methods
Tang Dhummakupt
ReplyDeleteHansen
1. A microfluidic device can be used to mix very precise amounts of reagents to help with crystallography techniques.
2. How does the mixer counteract the low Reynolds numbers in the device so easily?
3. How to derive protein structure from crystallography data
Patrick Diggins
ReplyDeleteHansen
1. The characterization of protein solubility can be implementation using complex processing at the nanoliter scale.
2. Why have computational approaches and attempts at to correlate measurements not been generally applicable?
3. How do chemical conditions and phase behavior affect the quality of crystals?
Parker Gould
ReplyDeleteHansen
1. The authors have created a MF device that can easily and automatically mix a large number of fluids. They used it to study the phase behavior of a protein.
2. How do the MF multiplexers work?
3. More on using LabVIEW to automate stuff.
4. Good demonstration of the power of MF
Liwei Jiang
ReplyDeleteHansen
1. The development of a microfluidics device that delivers computerized combinatorial mixing of solution reagents greatly simplifies protein crystallization.
2. What are some limitations of PCI? (For example, does the fidelity of the mixed solution suffer in a corner of the matrix?)
3. Show an actual protein phase diagram.
Stephen Arndt
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1. A microfluidic device was fabricated able to determine the phase partitioning of a protein via automated experiments that will be able devise a good strategy for further studies, ie crystallography
2. what is the time required for mixing in the device, I didn't see time courses for any of the experiments
3. methods of protein structure determination
Samat Kabani
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1. A microfluidic device was created that combined mixtures of 32 reagents in nanoliter volumes to improve crystallization in proteins.
2. Can the formulator chip be used for improving other desired characteristics other than crystallization or would the device have to be modified?
3. New developments on the "Lab on a Chip" concept
Rachel Harvey
ReplyDeleteHansen
1.Crystallization methods can be improved by using a formulation chip that can expedite the process of testing many reagents for characterizing appropriate phase behavior for a protein.
2. Can you elaborate on phase behavior? What does it mean?
3. Factors affecting protein crystallization
Chaitanya Allamneni
ReplyDeleteHansen
1. A microfluidic device was developed that combined a mixture of 32 reagents in order to study protein crystallograph
2. I too don;t really understand the relationship between phase behavior and crystallization.
3. Background on protein crystallography, method
Rosie Korman
ReplyDeleteHansen
1. A microfluidic formulator chip can be used to generate a map of the phase behavior of a protein.
2. What is meant by 'phase behavior of proteins'?
3. Other applications for this type of MF formulator device