SyBBURE Research Group

JOURNAL CLUB BLOG

Sunday, March 21, 2010

Journal Club This Tuesday

Sorry for the late notice. But here is the paper for this Tuesday's Journal Club. Let me if the link doesn't work.


http://www.sciencemag.org/cgi/reprint/313/5793/1642.pdf
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11 comments:

  1. AnonymousMarch 22, 2010 at 10:24 AM

    Peter DelNero
    Betzig

    1. I learned a new method for imaging intracellular proteins at nano-scale resolution using the PALM technique, which produces super-resolved images based on the sum of Gaussian of the standard deviation of PSFs over many images, rather than the deviation of the PSF itself. The process involves the repeated activation, measurement, and bleaching of the fluorescent molecules
    2. This process took up to 12 hours to obtain the image. Is there any method that yields the same resolution in less time?
    3. Fluorescent protein imaging, molecular-scale imaging
    4. How does this paper relate to SyBBURE research? Is anyone doing microscopy at this level?

    ReplyDelete
  2. funny brainMarch 22, 2010 at 12:13 PM

    Niki Arinze
    Betzig
    1. Fluorescent imaging can be used to localize proteins in cells
    2.How do PA-FPs work? Is it that they don't lose their fluorescent abilities and you just "recharge" them with light? How did the inactivated, unbleached molecules get depleted?
    3.importance of localization studies
    4. This is way over my head

    ReplyDelete
  3. Tommy ByrdMarch 22, 2010 at 4:14 PM

    Tommy Byrd
    Betzig
    1. The PALM technique of fluorescent imaging enables near-moleular localization vectors to marked proteins
    2. What is the bleaching method? Can you explain "blinking" and why it is a problem in this kind of imaging?
    3. Near field, stimulated emission depletion, structured illumination and reversible saturable optical fluorescence transitions microscopy
    4. My head is also fairly wet, but I like it

    ReplyDelete
  4. rachel harveyMarch 22, 2010 at 8:30 PM

    Rachel Harvey
    Betzig
    1. A fluorescent imaging method termed PALM can be used to image intracellular proteins at the nanoscale.
    2. Can you elaborate on VLPs?
    3. Nanoscale imaging techniques

    ReplyDelete
  5. RosieMarch 22, 2010 at 8:45 PM

    Rosie Korman
    Betzig
    1. The authors were able to use photoactivation localization microscopy to image fluorescent proteins with near molecular-level resolution.
    2. What exactly is photobleaching?
    Why/how is this method better than transmission electron microscopy?
    3. Other imaging methods that can provide this level of resolution.

    ReplyDelete
  6. Stephen ArndtMarch 22, 2010 at 8:47 PM

    Stephen Arndt
    Betzig
    1. PALM imaing was used to statistically localize protein in a cell
    2. So pessimist stephen asks why are they certain the proteins imaged don't simply occupy similar thermodynamically favorable spaces in the focal planes used? or are they sure it not just statistically averaging places proteins are likely to bind since they might have a conformation that favors binding a protein that is not able to be imaged via fluorescence?
    3. Plasmonics

    ReplyDelete
  7. Tang DhummakuptMarch 22, 2010 at 8:52 PM

    Tang Dhummakupt
    Betzig
    1. A fluorescent tagging method has been developed to detect fluorescent protein in the nanometer range.
    2. Is there any way for this method to be used without the need to fix the cells?
    3. The uses of fluorescent proteins

    ReplyDelete
  8. HussainMarch 22, 2010 at 9:37 PM

    Hussain Jinnah
    Betzig
    1. The authors developed a novel method that utilizes photoactivatable fluorescent proteins for nanoscale imaging of intracellular proteins.
    2. Interesting article. Does it relate to your project in some way?
    3. You could briefly discuss the transfection method used to tag proteins with the fluorescent activation gene.

    ReplyDelete
  9. Samat KabaniMarch 23, 2010 at 1:04 AM

    Samat Kabani
    Betzig
    1. Nanoscale imaging of intracellular proteins is possible through PALM.
    2. Does SyBBURE have the capability to do this?
    3. Fluorescent Imaging of Proteins

    ReplyDelete
  10. Chaitanya AllamneniMarch 23, 2010 at 1:56 PM

    Chaitanya Allamneni
    Beizig

    1. A method was introduced to image intracellular proteins in which photoactivable fluorescent proteins were activated.

    2. CAn you discuss the bleaching process of the proteins

    3. Other techniques to image on a nanoscale level

    ReplyDelete
  11. LaurenApril 1, 2010 at 10:52 AM

    Lauren Kolski
    Betzig
    1. PALM fluorescent imaging technique can image intracellular proteins on a nanoscale
    2. Could the long amount of time it takes to do this result in less accurate data?
    3. fluorescent imaging of proteins, common applications

    ReplyDelete

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