Elizabeth Lillie
So I have no idea if I'm doing this right but here is my attempt at posting my pcr:
1.) Learned about Microcontact printing of Proteins and the advantages of this technique over previous methods.
2.) This might be a dumb question, but what exactly does printing proteins on subtrates do, how do they work, like in the applications mentioned in the introduction?
3.) Application of microcontact printing in a more specified bioassay
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PCR:
ReplyDelete1) Applications of soft lithography other than what we do in SyBBURE
2) Why do proteins retain their biological activity after microcontanct printing but not after solid-phase synthesis?
How are DNA microarrays printed?
3) Applications of micro contact printing.
Patrick Diggins
ReplyDeleteBernard
1. Micro-contact Printing can be used in patterning proteins which is efficient and has the opportunity to be expanded into a wide range of applications.
2. Why is it important to keep the time between the drying of the stamp and the printing of the proteins short?
Why can some proteins not survive adsorption processes at surfaces?
3. The different surfaces on which micro-contact printing can be performed.
4. Is this something that Viibre is working on or intends to work on?
Arinze, Niki
ReplyDeleteBernard, et al.
1. Learned about the use of microcontact printing of proteins
2. What would you use it for? How can this be considered a form of soft lithography?
3. The history of Microcontact printing (other techniques that this sprang out from)
4. Are you (Parker) doing mCP of proteins or something else?
Hey Guys I have lab during this week's journal club but here's the PCR
ReplyDeleteChaitanya Allamneni
Bernard
1. Microcontact printing is a simple and cost-effective method to pattern proteins.
2. Did Bernard really ever outline why microcontact printing is necessary? What is done with these protein stamps? Is it used to "trap" an array of proteins, like our microfluidic devices trap cells?
3. Other sort of molecules/biological units that have been patterned using this method.
4. Whats the future of this method in VIIBRE? Will we try to replace our photolith methods with this?
Loi Hoang
ReplyDeleteBernard
1. MicroCP have lots of applications and advantages such as cost.
2. What is the advantages of being able to print more than one layer onto another.
3. An experiment that applied this method.
4. Is the success rate high enough to be used efficiently?
Liwei Jiang
ReplyDeleteBernard
1. Learned: µCP is basically stamping proteins onto a surface.
2. Question: How does BSA block non-specific binding?
3. Presentation: show us any cool stuff that you might have done with µCP.
4. Thoughts: Is this an effective method to bind trypsin to glass?
1)Biomolecules such as lipids and proteins may be stamped onto substrates while keeping their biological activity.
ReplyDelete2)What is the maximum number of proteins that a stamp can convey at one time and why not more?
3)What are the practical applications of stamping using biomolecules other than proteins.
4) How does this relate to your research projects?
Ayeeshik Kole
ReplyDeleteBernard
1. Microcontact printing has not been explored extensively at all and may have very big potential when paired with microfluidic devices, as the process is simple and efficient.
2. How does the parallel strategy work?
3. This paper was published in 2000. What new progress has been made?
4. What are the implications of microcontact printing on the research done within SyBBURE and VIIBRE?
Microcontact printing is a successful way to stamp on biomolecules onto a solid substrate.
ReplyDeleteCan you explain indirect methods and active placement?
The improvements made to this procedure.
Jennifer Greene
ReplyDeleteBernard
1. How to utilize microcontact printing successfully to stamp proteins and lipids and other biological molecules.
2. What is the success rate and reproducibility of the stamps?
3. Is this the same method Parker is using for his microstamp work? If not a good presentation could be about what modifications have you made to better apply it to your research.
Your Name
ReplyDeleteBernard
1. Learned: microCP is stamping protein onto sample's surface, which is another way to pattern samples
2. Pressing: This may be dumb, but I am kind of confused at the process. A normal micron contact printing requires silicon wafer to be coated with resist then develop the resist and through strip and silanize the silicon then coat with PDMS and produce the pattern, so, how this microncontact skip the master making part, where as the figure shows the master still needs to be made.
3. Presentation: How this is differ than traditional microcontact printing.
4. Thoughts: Present traditional microcontact printing and this novel approach.
@Liwei: This is similar to one of my hw question from last year, and I can't remember what is the right answer, but I put down something like this: BSA is bond at the place where doesn't need to be bind. Base on my understanding, BSA is selectively bond to the region where other protein doesn't want to bind. And since BSA is there already, then other protein can't bind.
Bernard
ReplyDelete1. Microcontact printing is a cost-efficient technique for patterning proteins.
2. Has anyone been using this method in VIIBRE?
3. Examples of macromolecules that are typically patterned with this technique.
1. Microcontact printing can be used to stamp organic molecules with very high resolution.
ReplyDelete2. Would it be possible to combine micro-contact printing with microfluidic devices?
3. Past uses of microcontact printing