Here is the article for this weeks journal club: http://www.nature.com/nmeth/journal/v2/n9/full/nmeth784.html
Thanks,
Ayeeshik and Katherine
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V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
JOURNAL CLUB BLOG
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Welcome to the SyBBURE Journal Club
Rules:
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
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Trevan Locke
ReplyDeleteGroisman
1. The authors developed a cell culture device that is capable of forming high density colonies on-chip.
2. Is there a danger of this device collapsing or leaking a lot since there are so many channels, chambers, and capillaries so close together?
3. Applications that might need a device like this one.
4. Guessing someone is gonna try to use this to get more yeast onto a single device?
Liwei Jiang
ReplyDeleteGroisman
1. The authors present a microfluidic device consisting of a series of media-permeable cell chambers.
2. (1) How is the gauge pressure regulated? (2) Are the polystyrene beads similar in size to yeast cells? (3) How do the authors know that metabolic wastes are permeable and are being flushed out? [Note: I won't be able to make the Journal Club. Could you post your answers as a reply directly on this page? Thanks!]
3. Is this device better or worse than the multitudes of other single-cell microfluidic chambers that we have seen?
Will Matloff
ReplyDeleteGroisman
1. A microfluidic chemostat was created that can grow very high density colonies of cells.
2. What are the benefits to having colonies of such high cell density?
3. A presentation could be given on the use of microfluidics for cell culture.
Groisman
ReplyDelete1. A MF device was created that can culture and sustain high densities of yeast and E. coli.
2. So the high loading pressures (for the channel raising) don't affect the yeast cells? I'm kind of assuming the higher pressure is accompanied by a higher flow velocity.
3. Other novel loading procedures.
Erica Curtis
ReplyDeleteGroisman
1. The authors developed a microfluidic chemostat that can support the growth of and maintain high density colonies.
2.Does the increase gauge pressure cause any lasting damage to the device? What are the limitations on cell size? Does cell size/density effect diffuse or waste build-up? How effective is washing the waste away if there is no flow in the chambers?
3. Compare to other devices.
Peter DelNero
ReplyDeleteGroisman
1. A microfluidic device was fabricated to mimic chemostat conditions, especially to overcome to problem of low-nutrient supply in high cell density environments
2. a) Scale-up of laboratory chemostats from 1-10 liters to thousands of liters in industry is already very challenging. Will experiments conducted on these devices be useful for industrial applications, or is it simply for theoretical utility? I guess my question is ultimately how is this useful? Maybe this article will be cited in other articles to answer this question.
b) What conditions does the microfluidic chamber lack compared to industrial chemostats? i.e. shear stresses from propellers, etc.
3. Fundamentals of chemostats and industrial bioreactors
4. What are the applications for this device?