Here's the link to the paper in case you need it:
http://pubs.acs.org/doi/full/10.1021/la026394k
Subscribe to:
Post Comments (Atom)
skip to main |
skip to sidebar
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
SyBBURE Research Group
JOURNAL CLUB BLOG
Posts
Welcome to the SyBBURE Journal Club
Rules:
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Use the following format to when you enter your PreClass Reading Comment (PCRC) on each assignment
Your Name
Journal article first author's last name
1. Learned
2. Pressing ?
3. Presentation
4. Thoughts
Suggested Papers
An integrated digital microfluidic lab-on-a-chip for clinical diagnostics on human …
V Srinivasan, VK Pamula, RB Fair - Lab on a chip, 2004 - ncbi.nlm.nih.gov
Search This Blog
Funded by the Searle Systems Biology and Bioengineering Undergraduate Research Experience at Vanderbilt University
BLOG CONCEPT AND DESIGN BY AMANDA STARLING GOULD. 2009
Trevan Locke
ReplyDeleteBrock
1. The authors learned that mammalian cells are more likely to extend lamellipodia from acute angles.
2. What is the null hypothesis?
3. Explanation of how these cells migrate
Kevin Qin
ReplyDeleteBrock
1. It was determined that by confining mammalian cells in geometric shapes with sharp angles, lamellipodia and deposition of fibronectin fibrils were preferentially extended from the corners of the confined regions.
2. Why did the hexagonal cell in Figure 5 display a 7th region of FN fibril accumulation? If the cells were confined in polygons with more sides, would abnormalities like what occurred in the hexagon happen?
3. FN fibril accumulation in more complex polygons.
1.Lamellipodia activity increases at corners.
ReplyDelete2. How does Fig 2f and 3c have the cells protruding less than the null hypothesis?
3. Differences in migration between mammalian cells and other cells.
Brock
ReplyDelete1. Mammalian cells preferentially move and deposit ECM around the corners of previously deposited ECM.
2. Any idea about why some cases showed very little aggregation or extensions in specific corners? (e.g. Figure 4D or 4G)
3. Different methods of cellular migration
Liwei Jiang
ReplyDeleteBrock
1. Cells prefer to move toward corners when are stranded on ECM-adhesion islands.
2. Why do you think cells prefer corners for pseudopodia formation?
3. Reliability of microcontact printing.
Will Matloff
ReplyDeleteBrock
1. Cell lamellipodia were shown to extend at the corners when placed on fibronectin islands of varying geometries.
2. Could the preference for the corners be similar to the diffusion corner effect that we saw last week? Also, what geometries are formed in the ECM?
3. Further explanation of the biochemistry behind this movement and the current research being done on this topic.
This comment has been removed by the author.
ReplyDeleteJoe Scherrer
ReplyDeleteBrock
1. Mammalian cells preferentially extend lamellipodia from the corners of ECM geometries.
2. Any particular reason why the cells prefer corners?
3. Mechanisms and mode of cellular migration.
Lindsay Chatfield
ReplyDeleteBrock
1. When stranded on a geometric ECM island, mammalian cells will preferentially extend their lamellipodia and filopodia to the corners of the geometric shape.
2. "The surface characteristics of the terrain over which the cell moves also may influence the direction of migration." So, what about the corner surfaces makes the cells move toward the corners? What's so special about the corners?
3. Growth and development of neurons using chemorepellants and attractants.
Ayeeshik Kole
ReplyDeleteBrock
1. Mammalian cells use spatial cues to direct their ECM deposition.
2. Why are there distinct peaks at corners?
3. Directing cell migration
Chaitanya Allamneni
ReplyDeleteBrock
1. Cell extend their lamellipoda/extensions toward the corners when placed on islands of varying geometry.
2. Why do the cells extend to multiple corners, and not uniformly move to one side?
3. Further chemotaxis studies on this movement.